AUTHOR=Marti-Marimon Maria , Vialaneix Nathalie , Lahbib-Mansais Yvette , Zytnicki Matthias , Camut Sylvie , Robelin David , Yerle-Bouissou Martine , Foissac Sylvain 

TITLE=Major Reorganization of Chromosome Conformation During Muscle Development in Pig

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.748239

DOI=10.3389/fgene.2021.748239

ISSN=1664-8021

ABSTRACT=<p>The spatial organization of the genome in the nucleus plays a crucial role in eukaryotic cell functions, yet little is known about chromatin structure variations during late fetal development in mammals. We performed <italic>in situ</italic> high-throughput chromosome conformation capture (Hi-C) sequencing of DNA from muscle samples of pig fetuses at two late stages of gestation. Comparative analysis of the resulting Hi-C interaction matrices between both groups showed widespread differences of different types. First, we discovered a complex landscape of stable and group-specific Topologically Associating Domains (TADs). Investigating the nuclear partition of the chromatin into transcriptionally active and inactive compartments, we observed a genome-wide fragmentation of these compartments between 90 and 110 days of gestation. Also, we identified and characterized the distribution of differential <italic>cis</italic>- and <italic>trans</italic>-pairwise interactions. In particular, <italic>trans</italic>-interactions at chromosome extremities revealed a mechanism of telomere clustering further confirmed by 3D Fluorescence <italic>in situ</italic> Hybridization (FISH). Altogether, we report major variations of the three-dimensional genome conformation during muscle development in pig, involving several levels of chromatin remodeling and structural regulation.</p>