AUTHOR=Jin Xingyue , Liu Yanhui , Hou Zhimin , Zhang Yunfei , Fang Yunying , Huang Youmei , Cai Hanyang , Qin Yuan , Cheng Yan 

TITLE=Genome-Wide Investigation of SBT Family Genes in Pineapple and Functional Analysis of AcoSBT1.12 in Floral Transition

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.730821

DOI=10.3389/fgene.2021.730821

ISSN=1664-8021

ABSTRACT=<p>SBT (Subtilisin-like serine protease), a clan of serine proteolytic enzymes, plays a versatile role in plant growth and defense. Although <italic>SBT</italic> family genes have been obtained from studies of dicots such as Arabidopsis, little is known about the potential functions of SBT in the monocots. In this study, 54 pineapple <italic>SBT</italic> genes (<italic>AcoSBT</italic>s) were divided into six subfamilies and then identified to be experienced strong purifying selective pressure and distributed on 25 chromosomes unevenly. <italic>Cis</italic>-acting element analysis indicated that almost all <italic>AcoSBT</italic>s promoters contain light-responsive elements. Further, the expression pattern via RNA-seq data showed that different <italic>AcoSBT</italic>s were preferentially expressed in different above-ground tissues. Transient expression in tobacco showed that AcoSBT1.12 was located in the plasma membrane. Moreover, Transgenic Arabidopsis ectopically overexpressing <italic>AcoSBT1.12</italic> exhibited delayed flowering time. In addition, under the guidance of bioinformatic prediction, we found that AcoSBT1.12 could interact with AcoCWF19L, AcoPUF2, AcoCwfJL, Aco012905, and AcoSZF1 by yeast-two hybrid (Y2H). In summary, this study provided valuable information on pineapple <italic>SBT</italic> genes and illuminated the biological function of <italic>AcoSBT1.12</italic> in floral transition.</p>