AUTHOR=Wu Huijie , Zhou Jingyuan , Chen Songda , Zhu Lingyu , Jiang Mengjie , Liu Aiqun 

TITLE=Survival-Related lncRNA Landscape Analysis Identifies LINC01614 as an Oncogenic lncRNA in Gastric Cancer

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.698947

DOI=10.3389/fgene.2021.698947

ISSN=1664-8021

ABSTRACT=<p><bold>Background:</bold> Long non-coding RNAs (lncRNAs) reportedly play important roles in biomarker and tumorigenesis of gastric cancer (GC). This study aimed to determine the potential application of prognostic lncRNA signature and identified the role of <italic>LINC01614</italic> in carcinogenesis in GC.</p><p><bold>Material and Methods:</bold> Data accessed from the Cancer Genome Atlas database was used to construct a lncRNA signature. Joint effect analysis of the signature and clinical parameters was performed to verify the clinical value of the signature. Co-expression analysis was conducted for prognostic lncRNAs and protein-coding genes. Moreover, the relative expression of <italic>LINC01614</italic> was validated in GC tissues and cell lines. <italic>In vitro</italic> and <italic>in vivo</italic> experiments were conducted to analyze the biological functions of the newly identified gene in GC cells.</p><p><bold>Results:</bold> A seven-lncRNA (<italic>LINC01614</italic>, <italic>LINC01537</italic>, <italic>LINC01210</italic>, <italic>OVAAL</italic>, <italic>LINC01446</italic>, <italic>CYMP-AS1</italic>, and <italic>SCAT8</italic>) signature was identified as a promising prognostic signature in GC. Results indicated that the seven-lncRNA was involved in tumorigenesis and progression pathways. <italic>LINC01614</italic> expression was identified and found to be upregulated in GC tissues and cells. The study findings revealed that <italic>LINC01614</italic> promoted cell proliferation, migration, invasion, and epithelial-mesenchymal transition. Knockdown of <italic>LINC01614</italic> arrested cell cycle distribution at the G2/M phase. Further, <italic>LINC01614</italic> also promoted tumor growth <italic>in vivo.</italic></p><p><bold>Conclusion:</bold> We developed an independent seven-lncRNA biomarker for prognostic prediction and identified <italic>LINC01614</italic> as an oncogenic lncRNA in GC.</p>