AUTHOR=Wang Shuqing , Wang Yang , Wang Shouhua , Tong Huanjun , Tang Zhaohui , Wang Jiandong , Zhang Yongjie , Ou Jingmin , Quan Zhiwei 

TITLE=Long Non-coding RNA FIRRE Acts as a miR-520a-3p Sponge to Promote Gallbladder Cancer Progression via Mediating YOD1 Expression

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.674653

DOI=10.3389/fgene.2021.674653

ISSN=1664-8021

ABSTRACT=<sec><title>Objectives</title><p>The role of lncRNAs in gallbladder cancer (GBC) remains poorly understood. In this study, we explored the function of functional intergenic repeating RNA element (FIRRE) in GBC.</p></sec><sec><title>Materials and Methods</title><p>Whole transcriptome resequencing was performed in three pairs of GBC tissues and adjacent non-tumor tissues. lncRNA FIRRE expression was verified by real-time PCR. The function of FIRRE in GBC was evaluated by experiments <italic>in vitro</italic> and <italic>in vivo</italic>. The mechanism of FIRRE was investigated via fluorescent <italic>in situ</italic> hybridization, RNA pull-down, dual luciferase reporter assays, and RNA immunoprecipitation.</p></sec><sec><title>Results</title><p>FIRRE level was dramatically increased in GBC tissues compared to that in the adjacent non-tumor tissues. High expression of FIRRE was closely related to clinical stage and poor prognosis in GBC patients. Moreover, FIRRE remarkably enhanced proliferation and migration, and inhibited apoptosis of GBC cells. Mechanistically, FIRRE modulated YOD1 expression by sponging miR-520a-3p, thus contributing to the development of GBC.</p></sec><sec><title>Conclusion</title><p>Our data revealed that FIRRE might act as a novel mediator in GBC progression by sponging miR-520a-3p and regulating YOD1. FIRRE might be regarded as a potential diagnostic marker or target for GBC treatment.</p></sec>