AUTHOR=Jiang Lijuan , Wang Yanjun , Tang Huancheng , Li Xiangdong , Huang Chaowen , Liu Zhuowei , Zhou Fangjian , Wang Xiaolan , Li Yonghong 

TITLE=CircCA12 Promotes Malignant Process via Sponging miR-1184 and Upregulating RAS Family in Bladder Cancer

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.663982

DOI=10.3389/fgene.2021.663982

ISSN=1664-8021

ABSTRACT=<p>Circular RNAs (circRNAs) are a panel of non-coding RNAs that mediate the regulation of gene expression, as well as pathological responses. Nonetheless, the function and expression pattern of circRNAs in urinary bladder cancer (UBC) remain unclear. Herein, we examined the function of circCA12 in UBC development. qRT-PCR results demonstrated remarkable circCA12 upregulation in UBC cell lines, as well as tissues. CCK-8, colony formation, and xenograft assays were employed to determine the effect of circCA12 on UBC. Our data illustrated silencing circCA12 repressed the proliferation along with the colony-formation capability of UBC cells. The migration and metastasis potential of UBC cells were remarkably abated <italic>in vivo</italic>, as well as <italic>in vitro</italic> after transfection with si-cirCA12 or sh-circCA12. Moreover, luciferase reporter and RIP assays indicated that circCA12 binds to miRNA-1184 through sponging miRNA, thereby up-regulating the expression of RAS family genes (<italic>NRAS</italic>, <italic>KRAS</italic>, and <italic>HRAS</italic>). In conclusion, the circCA12/miRNA-1184/RAS family was identified as a regulatory axis in UBC progression.</p>