AUTHOR=Xu Wenying , Liu Tong , Zhang Huiying , Zhu Hong 

TITLE=Mungbean DIRIGENT Gene Subfamilies and Their Expression Profiles Under Salt and Drought Stresses

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.658148

DOI=10.3389/fgene.2021.658148

ISSN=1664-8021

ABSTRACT=<p><italic>DIRIGENT</italic> (<italic>DIR</italic>) genes are key players in environmental stress responses that have been identified in many vascular plant species. However, few studies have examined the <italic>VrDIR</italic> genes in mungbean. In this study, we characterized 37 <italic>VrDIR</italic> genes in mungbean using a genome-wide identification method. <italic>VrDIRs</italic> were distributed on seven of the 11 mungbean chromosomes, and chromosome three contained the most <italic>VrDIR</italic> genes, with seven members. Thirty-two of the 37 <italic>VrDIRs</italic> contained a typical <italic>DIR</italic> gene structure, with one exon; the conserved DIR domain (i.e., Pfam domain) occupied most of the protein in 33 of the 37 <italic>VrDIRs</italic>. The gene structures of <italic>VrDIR</italic> genes were analyzed, and a total of 19 distinct motifs were detected. <italic>VrDIR</italic> genes were classified into five groups based on their phylogenetic relationships, and 13 duplicated gene pairs were identified. In addition, a total of 92 <italic>cis</italic>-acting elements were detected in all 37 <italic>VrDIR</italic> promoter regions, and <italic>VrDIR</italic> genes contained different numbers and types of <italic>cis</italic>-acting elements. As a result, <italic>VrDIR</italic> genes showed distinct expression patterns in different tissues and in response to salt and drought stress.</p>