AUTHOR=Li Xiaolong , Wu Qingmin , Zhang Xiaoxue , Li Chong , Zhang Deyin , Li Guoze , Zhang Yukun , Zhao Yuan , Shi Zhaoguo , Wang Weimin , Li Fadi TITLE=Whole-Genome Resequencing to Study Brucellosis Susceptibility in Sheep JOURNAL=Frontiers in Genetics VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.653927 DOI=10.3389/fgene.2021.653927 ISSN=1664-8021 ABSTRACT=
Brucellosis is a zoonotic disease and a major public health problem. However, the genetic mechanism of brucellosis in sheep remains unclear. In this study, serum samples were collected from 6,358 sheep from the F2 population (Dorper sheep ♂ × Hu sheep ♀), and antibody levels were continuously measured at 14 days and 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 months after administration of brucellosis vaccine. Finally, 19 brucellosis-resistant group (BRG) sheep and 22 brucellosis-susceptible group sheep (BSG) were screened for whole-genome sequencing. Using the fixation index, Fisher’s exact test, and chi-square test, a total of 205 candidate SNP sites were identified. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis suggested that 138 candidate genes were significantly enriched in adherens junction (