AUTHOR=Yang Lingzhi , Qi Qi , Wang Jian , Song Chengchuang , Wang Yanhong , Chen Xi , Chen Hong , Zhang Chunlei , Hu Linyong , Fang Xingtang 

TITLE=MiR-452 Regulates C2C12 Myoblast Proliferation and Differentiation via Targeting ANGPT1

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.640807

DOI=10.3389/fgene.2021.640807

ISSN=1664-8021

ABSTRACT=<p>microRNAs are a kind of endogenous, non-coding, single-strand small RNA. They have been reported as an important regulatory factor in skeletal myogenesis. In this study, miR-452 was selected from RNA high-throughput sequencing data to explore its regulatory role in myogenesis. Functionally, miR-452 overexpression could promote C2C12 myoblast proliferation while inhibiting myogenic differentiation. On the contrary, inhibition of miR-452 could suppress C2C12 myoblast proliferation but accelerate myogenic differentiation. Bioinformatics analysis and dual luciferase report assays showed that <italic>Angiopoietin 1</italic> (<italic>ANGPT1</italic>), <italic>RB1</italic>, and <italic>CACNB4</italic> were the potential target genes of miR-452. To further confirm the target relationship between <italic>ANGPT1</italic>, <italic>RB1</italic>, and <italic>CACNB4</italic> with miR-452, the mRNA level and protein level of these genes were detected by using RT-qPCR and Western blot, respectively. Result analysis indicated that <italic>ANGPT1</italic> was a target gene of miR-452. In addition, knockdown of <italic>ANGPT1</italic> could obviously promote C2C12 myoblast proliferation but block their differentiation. In summary, these results demonstrated that miR-452 promoted C2C12 myoblast proliferation and inhibited their differentiation <italic>via</italic> targeting <italic>ANGPT1</italic>.</p>