AUTHOR=Sun Hui , Wang Yan , Jing Hao-Yu , Yang Xin-Yu , Shi Xin-Xiu , Zhang Jia-Hui , Yu Yuan-Xiu , Gao Li , Wang Xin-Yue , Li Wan-Hong , Yu Lei 

TITLE=Chaperonin-Containing TCP1 Subunit 6A Is a Prognostic Potential Biomarker That Correlates With the Presence of Immune Infiltrates in Colorectal Cancer

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.629856

DOI=10.3389/fgene.2021.629856

ISSN=1664-8021

ABSTRACT=<sec><title>Aims</title><p>Chaperonin-containing TCP1 subunit (CCT) 6A is an oncogenic 6th subunit of the CCT family. Nevertheless, not much is documented regarding its function in colorectal cancer (COAD). This investigation seeks to explore the role of <italic>CCT6A</italic> in the prognosis of COAD.</p></sec><sec><title>Main Methods</title><p>Sequencing data from the Gene Expression Omnibus (GEO) and Cancer Genome Atlas database (TCGA) were employed to analyze the expression of <italic>CCT6A</italic> and its involvement in various regulatory networks behind COAD. Oncomine and Gene Expression Profiling Interactive Analysis (GEPIA) analyzed Levels of expression and survival rates, while GEPIA was used to uncover further the functional networks that involved <italic>CCT6A</italic>. Database for Annotation, Visualization, and Integrated Discovery (DAVID) tools were used to interpret Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways. Evaluation of the expression levels of <italic>CCT6A</italic> in COAD samples was also verified via immunohistochemistry.</p></sec><sec><title>Key Findings</title><p>We found that the expression of <italic>CCT6A</italic> is up-regulated in COAD. <italic>CCT6A</italic> correlated with poor prognosis and decreased immune infiltrates such as CD4<sup>+</sup> T cells, B cells, and dendritic cells. <italic>CCT6A</italic> is increased in COAD patients. <italic>CCT6A</italic> is associated with several gene networks related to the DDX family and mismatch repair pathways.</p></sec><sec><title>Significance</title><p>Our data showed that data mining was able to uncover data regarding levels of <italic>CCT6A</italic> and its involvement in genetic regulating pathways in COAD.</p></sec>