AUTHOR=Shestibratov Konstantin A. , Baranov Oleg Yu. , Mescherova Eugenia N. , Kiryanov Pavel S. , Panteleev Stanislav V. , Mozharovskaya Ludmila V. , Krutovsky Konstantin V. , Padutov Vladimir E. 

TITLE=Structure and Phylogeny of the Curly Birch Chloroplast Genome

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.625764

DOI=10.3389/fgene.2021.625764

ISSN=1664-8021

ABSTRACT=<p>Curly birch [<italic>Betula pendula</italic> var. <italic>carelica</italic> (Merckl.) Hämet-Ahti] is a relatively rare variety of silver birch (<italic>B. pendula</italic> Roth) that occurs mainly in Northern Europe and northwest part of Russia (Karelia). It is famous for the beautiful decorative texture of wood. Abnormal xylogenesis underlying this trait is heritable, but its genetic mechanism has not yet been fully understood. The high number of potentially informative genetic markers can be identified through sequencing nuclear and organelle genomes. Here, the <italic>de novo</italic> assembly, complete nucleotide sequence, and annotation of the chloroplast genome (plastome) of curly birch are presented for the first time. The complete plastome length is 160,523 bp. It contains 82 genes encoding structural and enzymatic proteins, 37 transfer RNAs (tRNAs), and eight ribosomal RNAs (rRNAs). The chloroplast DNA (cpDNA) is AT-rich containing 31.5% of A and 32.5% of T nucleotides. The GC-rich regions represent inverted repeats IR1 and IR2 containing genes of rRNAs (<italic>5S</italic>, <italic>4.5S</italic>, <italic>23S</italic>, and <italic>16S</italic>) and tRNAs (<italic>trn</italic>V, <italic>trn</italic>I, and <italic>trn</italic>A). A high content of GC was found in rRNA (55.2%) and tRNA (53.2%) genes, but only 37.0% in protein-coding genes. In total, 384 microsatellite or simple sequence repeat (SSR) loci were found, mostly with mononucleotide motifs (92% of all loci) and predominantly A or T motifs (94% of all mononucleotide motifs). Comparative analysis of cpDNA in different plant species revealed high structural and functional conservatism in organization of the angiosperm plastomes, while the level of differences depends on the phylogenetic relationship. The structural and functional organization of plastome in curly birch was similar to cpDNA in other species of woody plants. Finally, the identified cpDNA sequence variation will allow to develop useful genetic markers.</p>