AUTHOR=Litvinova Maria M. , Khafizov Kamil , Korchagin Vitaly I. , Speranskaya Anna S. , Asanov Aliy Yu. , Matsvay Alina D. , Kiselev Daniil A. , Svetlichnaya Diana V. , Nuralieva Sevda Z. , Moskalev Alexey A. , Filippova Tamara V. 

TITLE=Association of CASR, CALCR, and ORAI1 Genes Polymorphisms With the Calcium Urolithiasis Development in Russian Population

JOURNAL=Frontiers in Genetics

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2021.621049

DOI=10.3389/fgene.2021.621049

ISSN=1664-8021

ABSTRACT=<p>Kidney stone disease is an urgent medical and social problem. Genetic factors play an important role in the disease development. This study aims to establish an association between polymorphisms in genes coding for proteins involved in calcium metabolism and the development of calcium urolithiasis in Russian population. In this case-control study, we investigated 50 patients with calcium urolithiasis (experimental group) and 50 persons lacking signs of kidney stone disease (control group). For molecular genetic analysis we used a previously developed gene panel consisting of 33 polymorphisms in 15 genes involved in calcium metabolism: <italic>VDR</italic>, <italic>CASR</italic>, <italic>CALCR</italic>, <italic>OPN</italic>, <italic>MGP</italic>, <italic>PLAU</italic>, <italic>AQP1</italic>, <italic>DGKH</italic>, <italic>SLC34A1</italic>, <italic>CLDN14</italic>, <italic>TRPV6</italic>, <italic>KLOTHO</italic>, <italic>ORAI1</italic>, <italic>ALPL</italic>, and <italic>RGS14.</italic> High-throughput target sequencing was utilized to study the loci of interest. Odds ratios and 95% confidence intervals were used to estimate the association between each SNP and risk of urolithiasis development. Multifactor dimensionality reduction analysis was also carried out to analyze the gene-gene interaction. We found statistically significant (unadjusted <italic>p</italic>-value &lt; 0.05) associations between calcium urolithiasis and the polymorphisms in the following genes: <italic>CASR</italic> rs1042636 (OR = 3.18 for allele A), <italic>CALCR</italic> rs1801197 (OR = 6.84 for allele A), and <italic>ORAI1</italic> rs6486795 (OR = 2.25 for allele C). The maximum OR was shown for AA genotypes in loci rs1042636 (<italic>CASR</italic>) and rs1801197 (<italic>CALCR</italic>) (OR = 4.71, OR = 11.8, respectively). After adjustment by Benjamini-Hochberg FDR we found only <italic>CALCR</italic> (rs1801197) was significantly associated with the risk of calcium urolithiasis development. There was no relationship between recurrent course of the disease and family history of urolithiasis in investigated patients. Thus we found a statistically significant association of polymorphism rs1801197 (gene <italic>CALCR</italic>) with calcium urolithiasis in Russian population.</p>