AUTHOR=Karami Asma M. , Ødegård Jørgen , Marana Moonika H. , Zuo Shaozhi , Jaafar Rzgar , Mathiessen Heidi , von Gersdorff Jørgensen Louise , Kania Per W. , Dalsgaard Inger , Nielsen Torben , Buchmann Kurt 

TITLE=A Major QTL for Resistance to Vibrio anguillarum in Rainbow Trout

JOURNAL=Frontiers in Genetics

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2020.607558

DOI=10.3389/fgene.2020.607558

ISSN=1664-8021

ABSTRACT=<p>Genetic selection of disease resistant fish is a major strategy to improve health, welfare and sustainability in aquaculture. Mapping of single nucleotide polymorphisms (SNP) in the fish genome may be a fruitful tool to define relevant quantitative trait loci (QTL) and we here show its use for characterization of <italic>Vibrio anguillarum</italic> resistant rainbow trout (<italic>Oncorhynchus mykiss</italic>). Fingerlings were exposed to the pathogen <italic>V. anguillarum</italic> serotype O1 in a solution of 1.5 × 10<sup>7</sup> cfu/ml and observed for 14 days. Disease signs appeared 3 days post exposure (dpe) whereafter mortality progressed exponentially until 6 dpe reaching a total mortality of 55% within 11 days. DNA was sampled from all fish – including survivors – and analyzed on a 57 k Affymetrix SNP platform whereby it was shown that disease resistance was associated with a major QTL on chromosome 21 (Omy 21). Gene expression analyses showed that diseased fish activated genes associated with innate and adaptive immune responses. The possible genes associated with resistance are discussed.</p>