AUTHOR=Shekhovtsov Sergei V. , Shipova Aleksandra A. , Poluboyarova Tatiana V. , Vasiliev Gennady V. , Golovanova Elena V. , Geraskina Anna P. , Bulakhova Nina A. , Szederjesi Tímea , Peltek Sergei E. 

TITLE=Species Delimitation of the Eisenia nordenskioldi Complex (Oligochaeta, Lumbricidae) Using Transcriptomic Data

JOURNAL=Frontiers in Genetics

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2020.598196

DOI=10.3389/fgene.2020.598196

ISSN=1664-8021

ABSTRACT=<p><italic>Eisenia nordenskioldi</italic> (Eisen, <xref ref-type="bibr" rid="B4">1879</xref>) is the only autochthonous Siberian earthworm with a large distribution that ranges from tundra to steppe and broadleaved forests. This species has a very high morphological, ecological, karyological, and genetic diversity, so it was proposed that <italic>E. nordenskioldi</italic> should be split into several species. However, the phylogeny of the complex was unclear due to the low resolution of the methods used and the high diversity that should have been taken into account. We investigated this question by (1) studying the diversity of the COI gene of <italic>E. nordenskioldi</italic> throughout its range and (2) sequencing transcriptomes of different genetic lineages to infer its phylogeny. We found that <italic>E. nordenskioldi</italic> is monophyletic and is split into two clades. The first one includes the pigmented genetic lineages widespread in the northern and western parts of the distribution, and the second one originating from the southern and southeastern part of the species' range and representing both pigmented and non-pigmented forms. We propose to split the <italic>E. nordenskioldi</italic> complex into two species, <italic>E. nordenskioldi</italic> and <italic>Eisenia</italic> sp. 1 (aff. <italic>E. nordenskioldi</italic>), corresponding to these two clades. The currently recognized non-pigmented subspecies <italic>E. n. pallida</italic> will be abolished as a polyphyletic and thus a non-natural taxon, while <italic>Eisenia</italic> sp. 1 will be expanded to include several lineages earlier recognized as <italic>E. n. nordenskioldi</italic> and <italic>E. n. pallida</italic>.</p>