AUTHOR=Wang Yunhe , Bao Yaning , Zheng Yancheng , Guo Ping’an , Peng Dingxiang , Wang Bo 

TITLE=Promoter PPSP1–5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana

JOURNAL=Frontiers in Genetics

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2020.553265

DOI=10.3389/fgene.2020.553265

ISSN=1664-8021

ABSTRACT=<p>Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named <italic>P<sub><italic>PSP1</italic></sub>-BnPSP-1</italic>) and analyzed the function of its truncated fragments in Arabidopsis. The results show that <italic>P<sub><italic>PSP1</italic></sub>-BnPSP-1</italic> can drive the GUS reporter gene to be specifically expressed in the veins of Arabidopsis. After hormone and simulated drought treatment of the independent Arabidopsis lines carrying <italic>P<sub><italic>PSP1</italic></sub>-BnPSP-1</italic> and its truncated fragments, only <italic>P<sub><italic>PSP</italic>1–5</sub>-BnPSP-1</italic> (−600 to −1 bp region of <italic>P<sub><italic>PSP1</italic></sub>-BnPSP-1</italic>) is stably expressed and exhibits phloem specificity. Our findings suggest that <italic>P<sub><italic>PSP</italic>1–5</sub>-BnPSP-1</italic> can be used as a phloem specific promoter for further research.</p>