AUTHOR=Liu Yun , Zhu Zeyao , Ho Idy H. T. , Shi Yujian , Li Jianzhen , Wang Xia , Chan Matthew T. V. , Cheng Christopher H. K. 

TITLE=Genetic Deletion of miR-430 Disrupts Maternal-Zygotic Transition and Embryonic Body Plan

JOURNAL=Frontiers in Genetics

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2020.00853

DOI=10.3389/fgene.2020.00853

ISSN=1664-8021

ABSTRACT=<p><italic>MiR-430</italic> is considered an important regulator during embryonic development, but genetic loss-of-function study is still lacking. Here we demonstrated that genetic deletion of the <italic>miR-430</italic> cluster resulted in developmental defects in cell movement, germ layer specification, axis patterning and organ progenitor formation in zebrafish. Transcriptome analysis indicated that the maternally provided transcripts were not properly degraded whereas the zygotic genome expressed genes were not fully activated in the <italic>miR-430</italic> mutants. We further found that a reciprocal regulatory loop exists between <italic>miR-430</italic> and maternally provided transcripts: the maternally provided transcripts (<italic>Nanog</italic>, <italic>Dicer1</italic>, <italic>Dgcr8</italic>, and <italic>AGOs</italic>) are required for <italic>miR-430</italic> biogenesis and function, whereas <italic>miR-430</italic> is required for the clearance of these maternally provided transcripts. These data provide the first genetic evidence that <italic>miR-430</italic> is required for maternal-zygotic transition and subsequent establishment of embryonic body plan.</p>