AUTHOR=Chiricosta Luigi , Silvestro Serena , Gugliandolo Agnese , Marconi Guya Diletta , Pizzicannella Jacopo , Bramanti Placido , Trubiani Oriana , Mazzon Emanuela 

TITLE=Extracellular Vesicles of Human Periodontal Ligament Stem Cells Contain MicroRNAs Associated to Proto-Oncogenes: Implications in Cytokinesis

JOURNAL=Frontiers in Genetics

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2020.00582

DOI=10.3389/fgene.2020.00582

ISSN=1664-8021

ABSTRACT=<p>The human Periodontal Ligament Stem Cells (hPDLSCs) exhibit self-renewal capacity and clonogenicity potential. The Extracellular Vesicles (EVs) secreted by hPDLSCs are particles containing lipids, proteins, mRNAs, and non-coding RNAs, among which microRNAs, that are important in intercellular communication. The purpose of this study was the analysis of the non-coding RNAs contained in the EVs derived from hPDLSCs using Next Generation Sequencing. Moreover, our data were enriched using bioinformatic tools. The analysis highlighted the presence of non-coding RNAs and five microRNAs: <italic>MIR24-2</italic>, <italic>MIR142</italic>, <italic>MIR335</italic>, <italic>MIR490</italic>, and <italic>MIR296</italic>. Our results show that these miRNAs target the genes classified in two terms of the Gene Ontology: “Ras protein signal transduction” and “Actin/microtubule cytoskeleton organization.” Noteworthy, the in-deep analysis of our EVs highlights that the miRNAs could be implicated in the silencing of proto-oncogenes involved in 12 different types of tumors.</p>