AUTHOR=Xia Xin , Ruan Yi , Li Boya , Yu Yansong , Kong Xiangbo , Zhuang Peilin , Wu Hong 

TITLE=The Long Non-coding RNA lnc-DMP1 Regulates Dmp1 Expression Through H3K27Ac Modification

JOURNAL=Frontiers in Genetics

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2020.00233

DOI=10.3389/fgene.2020.00233

ISSN=1664-8021

ABSTRACT=<p>Several long non-coding RNAs (lncRNAs) have been reported regulate the expression of neighbor protein-coding genes at post-transcriptional, transcriptional and epigenetic levels. <italic>Dmp1</italic> (<italic>Dentin matrix protein 1</italic>), encoding a non-collagenous extracellular matrix protein, plays an important role in dentin and bone mineralization. However, the transcriptional regulation of lncRNA on <italic>Dmp1</italic> has not been reported. In this study, we identified a novel lncRNA named lnc-DMP1, which is near the <italic>Dmp1</italic> gene region and undergoes remarkable changes during mandible development. lnc-DMP1 is co-localized and significantly expressed correlation with <italic>Dmp1</italic> in embryonic and postnatal mouse mandibles. In MC3T3-E1 cells, lnc-DMP1 positively regulates DMP1 expression and skeletal mineralization. Furthermore, lnc-DMP1 induces the promoter activity of <italic>Dmp1</italic> by modulating H3K27Ac enrichment in the <italic>Dmp1</italic> promoter. In conclusion, our results indicate that lnc-DMP1 is a novel lncRNA near the <italic>Dmp1</italic> gene region and regulates <italic>Dmp1</italic> expression by modulating the H3K27 acetylation level of <italic>Dmp1</italic> promoter.</p>