AUTHOR=Tang Boping , Wang Zhongkai , Liu Qiuning , Zhang Huabin , Jiang Senhao , Li Xinzheng , Wang Zhengfei , Sun Yue , Sha Zhongli , Jiang Hui , Wu Xugan , Ren Yandong , Li Haorong , Xuan Fujun , Ge Baoming , Jiang Wei , She Shusheng , Sun Hongying , Qiu Qiang , Wang Wen , Wang Qun , Qiu Gaofeng , Zhang Daizhen , Li Yongxin 

TITLE=High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution

JOURNAL=Frontiers in Genetics

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2019.01340

DOI=10.3389/fgene.2019.01340

ISSN=1664-8021

ABSTRACT=<p>As an important freshwater aquaculture species in China, the Chinese mitten crab (<italic>Eriocheir japonica sinensis</italic>) has high economic and nutritional value. However, limited genomic information is currently available for studying its basic development and genetic diversity. Here, we performed whole-genome sequencing on Oxford Nanopore Technologies Limited's platform using promethION. The assembled size of <italic>E. j.sinensis</italic> genome was approximately 1.27 Gb, which is close to the estimated size (1.19 Gb). Furthermore, based on assessment using Benchmarking Universal Single-Copy Orthologs (BUSCO) (<xref ref-type="bibr" rid="B37">Simao et al., 2015</xref>), 94.00% of the expected eukaryotic genes were completely present in the genome assembly. In addition, repetitive sequences accounted for ~61.42% of the assembled genome, and 22,619 protein-coding genes were annotated. Comparative genomics analysis demonstrated that the Chinese mitten crab diverged from <italic>Penaeus vannamei</italic> ~373.6 million years ago, with a faster evolution rate than shrimp. We anticipate that the annotated high-quality genome of <italic>E. j. sinensis</italic> will promote research on its basic development and evolution and make substantial contributions to comparative genomic analyses of crustaceans.</p>