AUTHOR=Xie Shang-Qian , Xing Jian-Feng , Zhang Xiao-Ming , Liu Zhao-Yu , Luan Mei-Wei , Zhu Jie , Ling Peng , Xiao Chuan-Le , Song Xi-Qiang , Zheng Jun , Chen Ying 

TITLE=N6-Methyladenine DNA Modification in the Woodland Strawberry (Fragaria vesca) Genome Reveals a Positive Relationship With Gene Transcription

JOURNAL=Frontiers in Genetics

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2019.01288

DOI=10.3389/fgene.2019.01288

ISSN=1664-8021

ABSTRACT=<p><italic>N</italic><sup>6</sup>-methyladenine (6mA) DNA modification has been detected in several eukaryotic organisms, where it plays important roles in gene regulation and epigenetic memory maintenance. However, the genome-wide distribution patterns and potential functions of 6mA DNA modification in woodland strawberry (<italic>Fragaria vesca</italic>) remain largely unknown. Here, we examined the 6mA landscape in the <italic>F. vesca</italic> genome by adopting single-molecule real-time sequencing technology and found that 6mA modification sites were broadly distributed across the woodland strawberry genome. The pattern of 6mA distribution in the long non-coding RNA was significantly different from that in protein-coding genes. The 6mA modification influenced the gene transcription and was positively associated with gene expression, which was validated by computational and experimental analyses. Our study provides new insights into the DNA methylation in <italic>F. vesca</italic>.</p>