AUTHOR=Jiu Songtao , Xu Yan , Wang Jiyuan , Wang Lei , Wang Shiping , Ma Chao , Guan Le , Abdullah Muhammad , Zhao Maoxiang , Xu Wenping , Ma Wenli , Zhang Caixi 

TITLE=Genome-Wide Identification, Characterization, and Transcript Analysis of the TCP Transcription Factors in Vitis vinifera

JOURNAL=Frontiers in Genetics

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2019.01276

DOI=10.3389/fgene.2019.01276

ISSN=1664-8021

ABSTRACT=<p>The TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL FACTORS (TCP) protein, belonging to a plant-specific transcription factors (TFs) family, participates in the control of plant growth and development by regulating cell proliferation. Until now, a comprehensive study of concerning the TCP gene family and their roles in grapevine (<italic>Vitis vinifera</italic> L.) has not been completed. Using bioinformatics approaches, 17 <italic>VvTCP</italic> genes were identified and further classified into two classes, designated class I (PCF subclass) and class II (CIN and CYC/TB1 subclass), which was further supported by exon-intron organizations and conserved motif analysis. Promoter analysis demonstrated that <italic>VvTCPs</italic> have numerous <italic>cis</italic>-acting elements related to plant growth and development, phytohormone, and abiotic/biotic stress responses. The singleton duplication of grapevine <italic>TCP</italic> genes contributed to this gene family expansion. The syntenic analyses among <italic>Vitis vinifera</italic>, <italic>Arabidopsis</italic>, and <italic>Oryza sativa</italic> showed that these genes located in corresponding syntenic blocks arose before the divergence of <italic>V. vinifera</italic>, <italic>Arabidopsis</italic>, and <italic>O. sativa</italic>. The expression levels of 17 <italic>VvTCPs</italic> were determined in different tissues and fruit developmental stages, and abscisic acid (ABA) treatment. Seventeen <italic>VvTCPs</italic> exhibited distinct tissue-specific expression patterns, potentially illustrating the functional divergence of <italic>VvTCPs</italic> in all tested tissues. Eleven <italic>VvTCPs</italic> were down-regulated in five berry developmental stages, while three <italic>VvTCPs</italic> were up-regulated. Additionally, many members were strongly modulated by ABA treatment, suggesting these <italic>VvTCPs</italic> have important and diverse regulatory roles in ABA treatment. Our results provide valuable information on the evolution and functions of the <italic>VvTCPs</italic>, pave the way for further functional verification of these <italic>VvTCPs</italic> in grapevine.</p>