AUTHOR=Federico Anthony , Karagiannis Tanya , Karri Kritika , Kishore Dileep , Koga Yusuke , Campbell Joshua D. , Monti Stefano TITLE=Pipeliner: A Nextflow-Based Framework for the Definition of Sequencing Data Processing Pipelines JOURNAL=Frontiers in Genetics VOLUME=10 YEAR=2019 URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2019.00614 DOI=10.3389/fgene.2019.00614 ISSN=1664-8021 ABSTRACT=

The advent of high-throughput sequencing technologies has led to the need for flexible and user-friendly data preprocessing platforms. The Pipeliner framework provides an out-of-the-box solution for processing various types of sequencing data. It combines the Nextflow scripting language and Anaconda package manager to generate modular computational workflows. We have used Pipeliner to create several pipelines for sequencing data processing including bulk RNA-sequencing (RNA-seq), single-cell RNA-seq, as well as digital gene expression data. This report highlights the design methodology behind Pipeliner that enables the development of highly flexible and reproducible pipelines that are easy to extend and maintain on multiple computing environments. We also provide a quick start user guide demonstrating how to setup and execute available pipelines with toy datasets.