AUTHOR=García-Angulo Aglaya , Merlo Manuel A. , Rodríguez María E. , Portela-Bens Silvia , Liehr Thomas , Rebordinos Laureana 

TITLE=Genome and Phylogenetic Analysis of Genes Involved in the Immune System of Solea senegalensis – Potential Applications in Aquaculture

JOURNAL=Frontiers in Genetics

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2019.00529

DOI=10.3389/fgene.2019.00529

ISSN=1664-8021

ABSTRACT=<p>Global aquaculture production continues to increase rapidly. One of the most important species of marine fish currently cultivated in Southern Europe is <italic>Solea senegalensis</italic>, reaching more than 300 Tn in 2017. In the present work, 14 Bacterial Artificial Chromosome (BAC) clones containing candidate genes involved in the immune system (<italic>b2m</italic>, <italic>il10</italic>, <italic>tlr3</italic>, <italic>tap1</italic>, <italic>tnf</italic>α, <italic>tlr8</italic>, <italic>trim25</italic>, <italic>lysg</italic>, <italic>irf5</italic>, <italic>hmgb2</italic>, <italic>calr</italic>, <italic>trim16</italic>, and <italic>mx</italic>), were examined and compared with other species using multicolor Fluorescence <italic>in situ</italic> Hybridization (mFISH), massive sequencing and bioinformatic analysis to determine the genomic surroundings and syntenic chromosomal conservation of the genomic region contained in each BAC clone. The mFISH showed that the groups of genes <italic>hmgb2-trim25-irf5-b2m</italic>; <italic>tlr3-lysg</italic>; <italic>tnfα-tap1</italic>, and <italic>il10-mx-trim16</italic> were co-localized on the same chromosomes. Synteny results suggested that the studied BACs are placed in a smaller number of chromosomes in <italic>S. senegalensis</italic> that in other species. Phylogenetic analyses suggested that the evolutionary rate of immune system genes studied is similar among the taxa studied, given that the clustering obtained was in accordance with the accepted phylogenetic relationships among these species. This study contributes to a better understanding of the structure and function of the immune system of the Senegalese sole, which is essential for the development of new technologies and products to improve fish health and productivity.</p>