AUTHOR=Liu Jikai , Gao Yongfeng , Tang Yunlai , Wang Dan , Chen XiaoMing , Yao Yinan , Guo Yaoling 

TITLE=Genome-Wide Identification, Comprehensive Gene Feature, Evolution, and Expression Analysis of Plant Metal Tolerance Proteins in Tobacco Under Heavy Metal Toxicity

JOURNAL=Frontiers in Genetics

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2019.00345

DOI=10.3389/fgene.2019.00345

ISSN=1664-8021

ABSTRACT=<p>Plant metal tolerance proteins (MTPs) comprise a family of membrane divalent cation transporters that play essential roles in plant mineral nutrition maintenance and heavy metal stresses resistance. However, the evolutionary relationships and biological functions of <italic>MTP</italic> family in tobacco remain unclear. In the present study, 26, 13, and 12 <italic>MTPs</italic> in three main <italic>Nicotiana</italic> species (<italic>N. tabacum</italic>, <italic>N. sylvestris</italic>, and <italic>N. tomentosiformis</italic>) were identified and designated, respectively. The phylogenetic relationships, gene structures, chromosome distributions, conserved motifs, and domains of NtMTPs were systematic analyzed. According to the phylogenetic features, 26 NtMTPs were classified into three major substrate-specific groups that were Zn-cation diffusion facilitators (CDFs), Zn/Fe-CDFs, and Mn-CDFs, and seven primary groups (1, 5, 6, 7, 8, 9, and 12). All of the NtMTPs contained a modified signature sequence and the cation_efflux domain, whereas some of them also harbored the ZT_dimer. Evolutionary analysis showed that <italic>NtMTP</italic> family of <italic>N. tabacum</italic> originated from its parental genome of <italic>N. sylvestris</italic> and <italic>N. tomentosiformis</italic>, and further underwent gene loss and expanded via one segmental duplication event. Moreover, the prediction of <italic>cis</italic>-acting elements (CREs) and the microRNA target sites of <italic>NtMTP</italic> genes suggested the diverse and complex regulatory mechanisms that control <italic>NtMTPs</italic> gene expression. Expression profile analysis derived from transcriptome data and quantitative real-time reverse transcription-PCR (qRT-PCR) analysis showed that the tissue expression patterns of <italic>NtMTPs</italic> in the same group were similar but varied among groups. Besides, under heavy metal toxicity, <italic>NtMTP</italic> genes exhibited various responses in either tobacco leaves or roots. 19 and 15 <italic>NtMTPs</italic> were found to response to at least one metal ion treatment in leaves and roots, respectively. In addition, NtMTP8.1, NtMTP8.4, and NtMTP11.1 exhibited Mn transport abilities in yeast cells. These results provided a perspective on the evolution of <italic>MTP</italic> genes in tobacco and were helpful for further functional characterization of <italic>NtMTP</italic> genes.</p>