AUTHOR=She Jiajie , Yan Hengyu , Yang Jiaotong , Xu Wenying , Su Zhen 

TITLE=croFGD: Catharanthus roseus Functional Genomics Database

JOURNAL=Frontiers in Genetics

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2019.00238

DOI=10.3389/fgene.2019.00238

ISSN=1664-8021

ABSTRACT=<p><italic>Catharanthus roseus</italic> is a medicinal plant, which can produce monoterpene indole alkaloid (MIA) metabolites with biological activity and is rich in vinblastine and vincristine. With release of the scaffolded genome sequence of <italic>C. roseus</italic>, it is necessary to annotate gene functions on the whole-genome level. Recently, 53 RNA-seq datasets are available in public with different tissues (flower, root, leaf, seedling, and shoot) and different treatments (MeJA, PnWB infection and yeast elicitor). We used in-house data process pipeline with the combination of PCC and MR algorithms to construct a co-expression network exploring multi-dimensional gene expression (global, tissue preferential, and treat response) through multi-layered approaches. In the meanwhile, we added miRNA-target pairs, predicted PPI pairs into the network and provided several tools such as gene set enrichment analysis, functional module enrichment analysis, and motif analysis for functional prediction of the co-expression genes. Finally, we have constructed an online croFGD database (<ext-link ext-link-type="uri" xlink:href="http://bioinformatics.cau.edu.cn/croFGD/" xmlns:xlink="http://www.w3.org/1999/xlink">http://bioinformatics.cau.edu.cn/croFGD/</ext-link>). We hope croFGD can help the communities to study the <italic>C. roseus</italic> functional genomics and make novel discoveries about key genes involved in some important biological processes.</p>