AUTHOR=Gonçalves Tássia Mangetti , de Almeida Regitano Luciana Correia , Koltes James E. , Cesar Aline Silva Mello , da Silva Andrade Sónia Cristina , Mourão Gerson Barreto , Gasparin Gustavo , Moreira Gabriel Costa Monteiro , Fritz-Waters Elyn , Reecy James M. , Coutinho Luiz Lehmann 

TITLE=Gene Co-expression Analysis Indicates Potential Pathways and Regulators of Beef Tenderness in Nellore Cattle

JOURNAL=Frontiers in Genetics

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2018.00441

DOI=10.3389/fgene.2018.00441

ISSN=1664-8021

ABSTRACT=<p>Beef tenderness, a complex trait affected by many factors, is economically important to beef quality, industry, and consumer’s palatability. In this study, RNA-Seq was used in network analysis to better understand the biological processes that lead to differences in beef tenderness. Skeletal muscle transcriptional profiles from 24 Nellore steers, selected by extreme estimated breeding values (EBVs) for shear force after 14 days of aging, were analyzed and 22 differentially expressed transcripts were identified. Among these were genes encoding ribosomal proteins, glutathione transporter ATP-binding cassette, sub-family C (CFTR/MRP), member 4 (<italic>ABCC4</italic>), and synaptotagmin IV (<italic>SYT4</italic>). Complementary co-expression analyses using Partial Correlation with Information Theory (PCIT), Phenotypic Impact Factor (PIF) and the Regulatory Impact Factor (RIF) methods identified candidate regulators and related pathways. The PCIT analysis identified ubiquitin specific peptidase 2 (<italic>USP2</italic>), growth factor receptor-bound protein 10 (<italic>GBR10</italic>), anoctamin 1 (<italic>ANO1</italic>), and transmembrane BAX inhibitor motif containing 4 (<italic>TMBIM4</italic>) as the most differentially hubbed (DH) transcripts. The transcripts that had a significant correlation with <italic>USP2</italic>, <italic>GBR10</italic>, <italic>ANO1</italic>, and <italic>TMBIM4</italic> enriched for proteasome KEGG pathway. RIF analysis identified microRNAs as candidate regulators of variation in tenderness, including <italic>bta-mir-133a-2</italic> and <italic>bta-mir-22</italic>. Both microRNAs have target genes present in the calcium signaling pathway and apoptosis. PIF analysis identified myoglobin (<italic>MB</italic>), enolase 3 (<italic>ENO3</italic>), and carbonic anhydrase 3 (<italic>CA3</italic>) as potentially having fundamental roles in tenderness. Pathways identified in our study impacted in beef tenderness included: calcium signaling, apoptosis, and proteolysis. These findings underscore some of the complex molecular mechanisms that control beef tenderness in Nellore cattle.</p>