AUTHOR=Romiguier Jonathan , Roux Camille 

TITLE=Analytical Biases Associated with GC-Content in Molecular Evolution

JOURNAL=Frontiers in Genetics

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2017.00016

DOI=10.3389/fgene.2017.00016

ISSN=1664-8021

ABSTRACT=<p>Molecular evolution is being revolutionized by high-throughput sequencing allowing an increased amount of genome-wide data available for multiple species. While base composition summarized by GC-content is one of the first metrics measured in genomes, its genomic distribution is a frequently neglected feature in downstream analyses based on DNA sequence comparisons. Here, we show how base composition heterogeneity among loci and taxa can bias common molecular evolution analyses such as phylogenetic tree reconstruction, detection of natural selection and estimation of codon usage. We then discuss the biological, technical and methodological causes of these GC-associated biases and suggest approaches to overcome them.</p>