AUTHOR=Wang Yue , Yang Qiuping , Wang Zhimin TITLE=The evolution of nanopore sequencing JOURNAL=Frontiers in Genetics VOLUME=5 YEAR=2015 URL=https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2014.00449 DOI=10.3389/fgene.2014.00449 ISSN=1664-8021 ABSTRACT=

The “$1000 Genome” project has been drawing increasing attention since its launch a decade ago. Nanopore sequencing, the third-generation, is believed to be one of the most promising sequencing technologies to reach four gold standards set for the “$1000 Genome” while the second-generation sequencing technologies are bringing about a revolution in life sciences, particularly in genome sequencing-based personalized medicine. Both of protein and solid-state nanopores have been extensively investigated for a series of issues, from detection of ionic current blockage to field-effect-transistor (FET) sensors. A newly released protein nanopore sequencer has shown encouraging potential that nanopore sequencing will ultimately fulfill the gold standards. In this review, we address advances, challenges, and possible solutions of nanopore sequencing according to these standards.