Sandra G. Gava
Larissa L. S. Scholte1
Guilherme Oliveira- 1Grupo de Genômica e Biologia Computacional, Centro de Pesquisas René Rachou, Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais, Fundação Oswaldo Cruz - FIOCRUZ, Belo Horizonte, Brazil
- 2Genetics Department, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
- 3Núcleo de Pesquisa em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, Brazil
- 4Departamento de Biodiversidade, Evolução e Meio Ambiente, ICEB, Universidade Federal de Ouro Preto, Ouro Preto, Brazil
Introduction
The use of heterologous expression systems and expression vectors provide a powerful tool for studying the cellular functions of specific genes in their natural cellular environment or in specialized host organisms (Porro et al., 2005). In this context, the gene of interest is cloned in combination with a suitable promoter in a heterologous host (Gräslund et al., 2008). This approach has special relevance in the study of parasitic diseases, such as Schistosomiasis, in which the functional characterization of the parasite is hampered specially because these organisms are not amenable to genetic manipulation and their entire life cycle cannot be cultured in vitro (Alrefaei et al., 2011; Suttiprapa et al., 2012; Liang et al., 2013). Although genetic manipulation it is difficult to be achieved in parasitic helminthes, significant advances have been reported towards development of transgenic schistosomes as gene silencing by RNA interference (RNAi) and transient and stable transfection including transgenesis mediated by genome integration using competent vectors or retrovirus (Beckmann and Grevelding, 2012; Mann et al., 2014). Therefore, protein expression in a heterologous host may help the understanding of physiological processes and identifying potential targets with biomedical and biotechnological applications.
Schistosoma mansoni is one of the causative agents of human intestinal schistosomiasis. The availability of the genome sequence and a significant amount of transcriptomic and proteomic information allowed the application of a variety of methodologies for the identification and characterization of molecules involved in different physiological mechanisms such as cell signaling essential for the S. mansoni's parasite biology (Knudsen et al., 2005; Curwen et al., 2006; Cass et al., 2007; Guillou et al., 2007).
Protein kinases (PK) play key roles in signaling pathways and have been proposed as potential targets for the development of new anti-schistosome drugs (Dissous et al., 2007). Approximately 1.9% (252 proteins) of the predicted S. mansoni proteome corresponds to PKs. However, less than 15% of the kinases have experimental functional evidence, including the JNK subfamily member (Andrade et al., 2011). In contrast to the number of homologs in other species, Andrade and colleagues (2011) have identified only one protein belonging to the JNK subfamily (Smp_172240) in the S. mansoni proteome. Knockdown of SmJNK by RNA interference (RNAi) in schistosomula significantly reduced the total numbers of eggs and adult parasites in infected mice (Andrade, 2012). In addition, worms recovered from infected mice showed underdeveloped tegument and reproductive organs (Andrade, 2012). These results indicate that SmJNK might play a role in parasite transformation and survival in the mammal host. However, this line of approach has one methodological limitation. In schistosomes it is not yet possible to conduct functional complementation experiments. In order to advance our knowledge concerning SmJNK gene function we explored the nematode Caenorhabditis elegans as a heterologous platform to investigate whether S. mansoni JNK represents a physiologically functional protein.
C. elegans is a transparent, free-living, soil nematode with 1 mm in length that has emerged as an important animal model in various fields including neurobiology, developmental biology, and genetics. C. elegans was the first genome of a multicellular organism to be fully sequenced (The C. elegans Sequencing Consortium, 1998). This model offers several advantages, including well-established techniques for genetic and experimental manipulation. Transformation of C. elegans has been used to investigate the function of genes from a range of parasitic nematode species, including tub-1 and cpl-1 from Haemonchus contortus, gst-3 from Onchocerca volvulus and fktf-1b from Strongyloids stercoralis (Grant, 1992; Kwa et al., 1995; Britton et al., 1999; Redmond et al., 2001; Britton and Murray, 2002; Kampkötter et al., 2003; Massey et al., 2006).
The C. elegans genome encodes five proteins classified as belonging to the subfamily JNK (jnk-1, ZC416.4, T07A9.3, Y51B9A.9, and C49C3.10). Based on evolutionary relationships of the JNK protein, C. elegans jnk-1 was found to be the orthologue of SmJNK (data not shown). In C. elegans, jnk-1 is involved in the modulation of coordinated locomotion (Villanueva et al., 2001). C. elegans jnk-1 deletion mutants are short-lived, more susceptible to heavy metal and heat stress (Villanueva et al., 2001; Wolf et al., 2008). Overexpression of jnk-1 increases the resistance to oxidative stress and prolongs the worm's lifespan (Villanueva et al., 2001; Oh et al., 2005). Based on this information, we tested whether the overexpression of SmJNK in C. elegans would also result in similar phenotypes, enabling the demonstration that the schistosome enzyme is active and capable of complementing the function of the original gene.
Strain Construction
The expression vector must contain all the DNA sequences necessary for its own expression. The C. elegans transcription machinery should be able to recognize and correctly interpret the signals present in these sequences. In order to obtain specific transgenic lineages overexpressing SmJNK, we first constructed the expression cassettes containing the cDNA of S. mansoni SmJNK (Sm_JNK) downstream of the C. elegans jnk-1 gene promoter (Ce_jnk-1p).
We have chosen to use the C. elegans promoter because we had no knowledge if the C. elegans transcription machinery would recognize the S. mansoni promoter. Additionally, it is possible that gene expression patterns may be differente in two species and the use of the S. mansoni promoter may produce a different phenotype due to expression in different cell types or at different levels (Cook et al., 2006). The systematic study of schistosome gene promoters is an area not much explored, but of central relevance for transgenese studies in the field. In C. elegans, the majority of protein coding genes are within gene-dense regions of the genome, with cis-acting regulatory regions usually close to the coding region. Consequently, the minimal promoter region required for proper expression of most RNA Polymerase II transcripts lies within a couple of kilobases upstream of the start codon (Okkema and Krause, 2005). For this reason, we selected the 3 Kb region upstream of Ce_jnk-1 gene as a promoter region. We used S. mansoni cDNA to amplify the coding regions to avoid any possibility of incorrect splicing, once its intronic regions could not be recognized by the C. elegans splicing machinery. As a positive control, we also construct another transgenic line containing jnk-1 cDNA of C. elegans (Ce_JNK-1) under control of the same promoter Ce_jnk-1p. The 3'-UTR region was not included due to the lack of exclusive restriction sites in this region.
The DNA final constructs were delivered to C. elegans N2 through intragonadal microinjections (Mello et al., 1991). As previous described by Oh et al. (2005) (Figure 1A), the plasmids were injected at 50 ng/μL into the gonad of young adult N2 worms to generate stable extrachromosomal transgenic lines. Plasmid pRF4 [rol-6(su1066) plasmid], which has a dominant mutation in rol-6, was coinjected at 100 ng/μL for selection by the induction of a dominant “roller” phenotype in the transgenic lineages (Figure 1A). We generated three independent extrachromosomal lineages for Ce_JNK-1 (N2 Ex01[Ce_jnk-1p::Ce_JNK-1], N2 Ex02[Ce_jnk-1p::Ce_JNK-1] and N2 Ex03[Ce_jnk-1p::Ce_JNK-1]) and two independent extrachromosomal lineages expressing Sm_JNK (N2 Ex04[Ce_jnk-1p::Sm_JNK] and N2 Ex05[Ce_jnk-1p::Sm_JNK]). We used wild type worms microinjected only with the plasmid pRF4 as negative control. All strains were maintained on nematode growth medium (NGM) plates at 20°C and fed with bacteria of the E. coli OP50 strain, as described by Brenner (1974).
Figure 1. Experimental cloning strategy and analysis of the expression level and the longevity in C. elegans overexpression Sm_JNK. (A) The promoter region of Ce_jnk-1p gene was amplified from genomic DNA of adult C. elegans N2 Bristol by PCR (5'-GCGCGCAAACTTCCATCTCCTGTTTCTC, 3'-GCGCGCGTGCACAGGATCACACACTTTA). Total RNA was extracted from schistosomula and C. elegans adult worms using TRIzol® reagent (Invitrogen) following the standard manufacturer's protocol. CDS of Sm_JNK (5'-GCGGCCGCATGGCAAACAACATTCCTCC, 3'-GTCGACTTAATTTTGAATATTACGTA) and Ce_JNK-1 (5'-GCGGCCGCATGGAGGAACGATTATCCAC, 3'GTCGACTCAGGAATAAATGTCATGGG) were amplified from synthesized cDNA by PCR. Fragments were subsequently cloned in pGEM®-T vector. The construction obtained was digested with restriction enzymes (NotI and SalI) to linearize the vector containing the promoter region and to recover the CDS. Subsequently, subcloning was performed by ligation of C. elegans and S. mansoni CDS with the construct containing the promoter region. The plasmids were injected at 50 ng/μL into the gonad of young adult N2 worms to generate stable extrachromosomal transgenic lines. mRNA level of Ce_JNK-1 (B) Sm_JNK (C) in wild-type animals. mRNA levels were measured in the transgenic lineages obtained for Ce_JNK-1 (N2 Ex01[Ce_jnk-1p::Ce_JNK-1], N2 Ex02[Ce_jnk-1p::Ce_JNK-1], and N2 Ex03[Ce_jnk-1p::Ce_JNK-1]) and lineages obtained for Sm_JNK (N2 Ex04[Ce_jnk-1p::Sm_JNK] and N2 Ex05[Ce_jnk-1p::Sm_JNK]). Total RNA of each transgenic lines or N2 worms was isolated from approximately 50 animals using TRIzol® reagent (Invitrogen). cDNA was synthesized using SuperScript™ II Reverse Transcriptase (Invitrogen). RT-qPCR was performed in triplicate with a ABI 7500 RT-PCR system (Applied Biosystems) using SYBR® Green (Applied Biosystems) and the data was analyzed using the comparative Ct method (Livak and Schmittgen, 2001). Relative mRNA levels were normalized to cdc-42 mRNA levels. (D) Worms in L4 stage or young adults were then transferred to new NGM plates containing 0.1 mg/mL 5'-flurodeoxyuridine (FUDR) to prevent progeny growth (Hosono et al., 1982). Animals were tapped every two days and scored as dead when they did not respond to the platinum wire pick. We determined worm's survival from the point when they were transferred to the FUDR plate and lifespan was defined as the account of days that the worms survived starting at day 1. The lifespan assays were repeated three times and statics analysis were done using the Log-rank (Mantel-Cox) test.
Expression Levels and Lifespan Analysis
Expression of the Ce_JNK-1 and Sm_JNK was detected in all transgenic lines (Figures 1B,C). However, expression levels of the transgene varied substantially among. In the transgenic lines obtained in our study, the expression level of Ce_JNK-1 ranged from 30 times in the strain Ce_JNK-1 Ex01 to 1.5 times in the strain N2 Ce_JNK-1 Ex03 when compared with the control lineage (Figure 1B). The expression level of Sm_JNK also varied ranging from 40 times in the strain Sm_JNK Ex04 to 11 times in the strain Sm_JNK Ex04 in comparison to the control line (Figure 1C). Any regulatory factor present in cis introns were absent in our constructions, once constructions were synthesized from cDNA. It is possible that the addition of such region could further increase the level of expression observed.
Next, we evaluated whether the Sm_JNK overexpression could increase the C. elegans lifespan (Figure 1D). To monitor longevity under normal conditions, Ce_JNK-1 and Sm_JNK animals were grown at 20°C and scored every two days. Despite the increased expression of Ce_JNK-1 in transgenic lineages, we did not observe any increase in their longevity as earlier described by Oh et al. (2005). We also did not observe any phenotypic changes in transgenic lines overexpressing Sm_JNK. These results could be explained by the absence of introns or 3'-UTR region in the constructs used in this work. As previously described in the literature, the success of phenotype rescue experiments depends on factors such as the presence of regulatory elements in promoter region, the correct processing of cis-, and possibly trans-splicing, as well as 3' formation of the pre-RNA to produce mature mRNA (Gilleard, 2004). Moreover, the steady-state levels of proteins in eukaryotic cells are also strongly dependent on translational regulatory mechanisms. The overall rate of protein synthesis as well as the translational efficiencies of individual mRNAs are regulated in response to different signals. Therefore, the over expression observed at mRNA level does not may necessarily result in increased protein levels in the correct active form. It is also worth mentioning that the microinjection technique used to obtain transgenic lines carrying repetitive extrachromosomal arrays is relatively fast and efficient. However, one of its drawbacks is that it is difficult to predict and control the level of expression among different arrays resulting in strains with distinct levels of transgene expression. Furthermore, the DNA is injected in the target tissue in an established concentration, but it is not possible to control the amount of DNA successfully injected into each gonad, nor the amount incorporated into arrays in each strain obtained (Evans, 2006).
Conclusions
Heterologous expression experiments have been performed an alternative approach to characterize schistosome genes. In the present paper, we described for the first time the use of C. elegans as an alternative heterologous host to functional studies in S. mansoni. The nematode C. elegans is more closely related to S. mansoni than bacteria, yeast, protozoa, or mammal cells, some of which have been used in heterologous species experiments. The excellent assembly and annotation of the C. elegans genome sequence is a valuable resource for studying the developmental and functional biology of parasites.
In addition to the technical difficulties, there are problems regarding to the functional extrapolation of a parasite gene expressed in transgenic C. elegans. As all heterologous expression system, one must be careful when extrapolating data, particularly in the functional analysis of distantly related species, which genes conserved in sequence level may be involved in different biological activities (Britton and Murray, 2002). Thus, the ability of a gene from another species to rescue a phenotype in a C. elegans mutant does not necessarily imply in a relationship of orthology or does it mean that these genes function in the same way and in similar pathways in both species. Likewise, the failure to recover a phenotype does not necessarily suggest that the genes are involved in different processes. Since there are mechanisms for co-evolution between molecules, genes with similar functions in similar pathways cannot perform its function, because it does not interact with their downstream targets in C. elegans (Gilleard, 2004). Nevertheless, the use of C. elegans still provides a conceptual and practical framework for functional studies of parasite genes.
An alternative approach to improve the success of S. mansoni heterologous expression experiments using C. elegans as host would be to perform microinjection using constructs containing: (i) coding regions cloned into vectors specific for expression in C. elegans; (ii) synthetically constructed DNA containing the promoter region, the coding region, and 3'-UTR region of the gene of interest.
We hope that the results obtained in this study will contribute to the designing of future experiments that intend to use heterologous expression as an alternative approach to functional studies in S. mansoni.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
The authors thank Dr. Luciano Andrade Moreira (CPqRR) for providing the microinjector and the dependencies of the Malaria laboratory and Dr. Marina de Moraes Mourão (CPqRR) and Dr. Rosiane A. da Silva Pereira (CPqRR) for valuable assistance and helpful scientific discussions. The authors are also grateful to Francislon Silva (CPqRR) for bioinformatics technical support and to Mariana de Oliveira (CPqRR) for help with illustrations. This work received support from the CNPq, INCT-DT and CPqRR-FICOCRUZ/MG.
References
Alrefaei, Y. N., Okatcha, T. I., Skinner, D. E., and Brindley, P. J. (2011). Progress with schistosome transgenesis. Memórias Do Instituto Oswaldo Cruz 106, 785–793.
Andrade, L. F. (2012). Proteínas Quinase Eucarióticas (ePKs) de Schistosoma Ssp: Identificação, Anotação Funcional E Seleção de Alvos Para O Desenvolvimento de Droga. Belo Horizonte, MG: Centro de Pesquisas René Rachou.
Andrade, L. F., Nahum, L. A., Avelar, G. A., Silva, L. L., Zerlotini, A., Ruiz, J. C., et al. (2011). Eukaryotic protein kinases (ePKs) of the helminth parasite Schistosoma mansoni. BMC Genomics 12:215. doi: 10.1186/1471-2164-12-215
Beckmann, S., and Grevelding, C. G. (2012). Paving the way for transgenic schistosomes. Parasitology 139, 651–668. doi: 10.1017/S0031182011001466
Britton, C., Redmond, D. L., Knox, D. P., McKerrow, J. H., and Barry, J. D. (1999). Identification of Promoter Elements of Parasite Nematode Genes in Transgenic Caenorhabditis Elegans. Mol. Biochem. Parasitol. 103, 171–181.
Britton, C., and Murray, L. (2002). A Cathepsin L protease essential for Caenorhabditis Elegans embryogenesis is functionally conserved in parasitic nematodes. Mol. Biochem. Parasitol. 122, 21–33. doi: 10.1016/S0166-6851(02)00066-X
Cass, C. L., Johnson, J. R., Califf, L. L., Xu, T., Hernandez, H. J., Stadecker, M. J., et al. (2007). Proteomic analysis of schistosoma mansoni egg secretions. Mol. Biochem. Parasitol. 155, 84–93. doi: 10.1016/j.molbiopara.2007.06.002
Cook, A., Aptel, N., Portillo, V., Siney, E., Sihota, R., Holden-Dye, L., et al. (2006). Caenorhabditis Elegans ivermectin receptors regulate locomotor behaviour and are functional orthologues of haemonchus contortus receptors. Mol. Biochem. Parasitol. 147, 118–125. doi: 10.1016/j.molbiopara.2006.02.003
Curwen, R. S., Ashton, P. D., Sundaralingam, S., and Wilson, R. A. (2006). Identification of novel proteases and immunomodulators in the secretions of schistosome cercariae that facilitate host entry. Mol. Cell. Proteom. 5, 835–844. doi: 10.1074/mcp.M500313-MCP200
Dissous, C., Ahier, A., and Khayath, N. (2007). Protein tyrosine kinases as new potential targets against human schistosomiasis. BioEssays 29, 1281–1288. doi: 10.1002/bies.20662
Evans, T. (2006). Transformation and microinjection. WormBook: 1–15. doi: 10.1895/wormbook.1.108.1. Available online at: http://www.wormbook.org/chapters/www_transformationmicroinjection/transformationmicroinjection.html
Gilleard, J. S. (2004). The Use of Caenorhabditis Elegans in parasitic nematode research. Parasitology 128, S49–S70. doi: 10.1017/S003118200400647X
Grant, W. N. (1992). Transformation Os Caenorhabditis Elegans with genes from parasitic nematodes. Parasitol. Today 8, 344–346.
Gräslund, S., Nordlund, P., Weigelt, J., Hallberg, B. M., Bray, J., Gileadi, O., Knapp, S., et al. (2008). Protein production and purification. Nat. Methods 5, 135–146. doi: 10.1038/nmeth.f.202
Guillou, F., Roger, E., Moné, Y., Rognon, A., Grunau, C., Théron, A., et al. (2007). Excretory-secretory proteome of larval schistosoma mansoni and echinostoma caproni, two parasites of biomphalaria glabrata. Mol. Biochem. Parasitol. 155, 45–56. doi: 10.1016/j.molbiopara.2007.05.009
Hosono, R., Mitsui, Y., Sato, Y., Aizawa, S., and Miwa, J. (1982). Life span of the wild and mutant nematode Caenorhabditis Elegans. Effects of sex, sterilization, and temperature. Exp. Gerontol. 17, 163–172.
Kampkötter, A., Volkmann, T. E., de Castro, S. H., Leiers, B., Klotz, L. O., Johnson, E. T., et al. (2003). Functional analysis of the glutathione S-Transferase 3 from Onchocerca Volvulus (Ov-GST-3): a parasite GST confers increased resistance to oxidative stress in Caenorhabditis Elegans. J. Mol. Biol. 325, 25–37. doi: 10.1016/S0022-2836(02)01174-9
Knudsen, G. M., Medzihradszky, K. F., Lim, K. C., Hansell, E., and McKerrow, J. H. (2005). Proteomic Analysis of schistosoma mansoni cercarial secretions. Mol. Cell. Proteom. 4, 1862–1875. doi: 10.1074/mcp.M500097-MCP200
Kwa, M. S., Veenstra, J. G., Van Dijk, M., and Roos, M. H. (1995). Beta-tubulin genes from the parasitic nematode haemonchus contortus modulate drug resistance in Caenorhabditis Elegans. J. Mol. Biol. 246, 500–510. doi: 10.1006/jmbi.1994.0102
Liang, S., Knight, M., and Jolly, E. R. (2013). Polyethyleneimine mediated DNA transfection in schistosome parasites and regulation of the WNT signaling pathway by a dominant-negative SmMef2. PLoS Negl. Trop. Dis. 7:e2332. doi: 10.1371/journal.pntd.0002332
Livak, K. J., and Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402–408. doi: 10.1006/meth.2001.1262
Mann, V. H., Suttiprapa, S., Skinner, D. E., Brindley, P. J., and Rinaldi, G. (2014). Pseudotyped murine leukemia virus for schistosome transgenesis: approaches, methods and perspectives. Transgenic Res. doi: 10.1007/s11248-013-9779-3. [Epub ahead of print].
Massey, H. C., Bhopale, M. K., Li, X., Castelletto, M., and Lok, J. B. (2006). The fork head transcription factor FKTF-1b from Strongyloides Stercoralis restores DAF-16 developmental function to mutant Caenorhabditis Elegans. Int. J. Parasitol. 36, 347–352. doi: 10.1016/j.ijpara.2005.11.007
Mello, C. C., Kramer, J. M., Stinchcomb, D., and Ambros, V. (1991). Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J. 10, 3959–3970.
Oh, S. W., Mukhopadhyay, A., Svrzikapa, N., Jiang, F., Davis, R. J., and Tissenbaum, H. A. (2005). JNK regulates lifespan in Caenorhabditis Elegans by modulating nuclear translocation of forkhead transcription factor/DAF-16. Proc. Natl. Acad. Sci. U.S.A. 102, 4494–4499. doi: 10.1073/pnas.0500749102
Okkema, P. G., and Krause, M. (2005). Transcriptional Regulation. Wormbook 23, 1–40. doi: 10.1895/wormbook.1.45.1
Porro, D., Sauer, M., Branduardi, P., and Mattanovich, D. (2005). Recombinant protein production in yeasts. Mol. Biotechnol. 31, 245–259. doi: 10.1007/978-1-61779-433-9_17
Redmond, D. L., Clucas, C., Johnstone, I. L., and Knox, D. P. (2001). Expression of haemonchus contortus pepsinogen in Caenorhabditis Elegans. Mol. Biochem. Parasitol. 112, 125–131. doi: 10.1016/S0166-6851(00)003571-1
Suttiprapa, S., Rinaldi, G., and Brindley, P. J. (2012). Genetic manipulation of schistosomes–progress with integration competent vectors. Parasitology 139, 641–650. doi: 10.1017/S003118201100134X
The C. elegans Sequencing Consortium, D. D. (1998). Genome sequence of the nematode C. Elegans: a platform for investigating biology. Science 282, 2012–2018. doi: 10.1126/science.282.5396.2012
Villanueva, A., Lozano, J., Morales, A., Lin, X., Deng, X., Hengartner, M. O., et al. (2001). Jkk-1 and Mek-1 regulate body movement coordination and response to heavy metals through Jnk-1 in Caenorhabditis Elegans. EMBO J. 20, 5114–5128. doi: 10.1093/emboj/20.18.5114
Keywords: Schistosoma mansoni, heterologous expression, Caenorhabditis elegans
Citation: Gava SG, Scholte LLS, Volpini Â, de Paula Oliveira R and Oliveira G (2014) Heterologous expression in Caenorhabditis elegans as an alternative approach to functional studies in Schistosoma mansoni. Front. Genet. 5:120. doi: 10.3389/fgene.2014.00120
Received: 12 February 2014; Accepted: 18 April 2014;
Published online: 27 May 2014.
Edited by:
Arnon Dias Jurberg, Oswaldo Cruz Institute/Oswaldo Cruz Foundation (Fiocruz), BrazilReviewed by:
Gabriel Rinaldi, The George Washington University, USAEmmitt Randolph Jolly, Case Western Reserve University, USA
Copyright © 2014 Gava, Scholte, Volpini, de Paula Oliveira and Oliveira. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: oliveira@cpqrr.fiocruz.br