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ORIGINAL RESEARCH article

Front. For. Glob. Change
Sec. Pests, Pathogens and Invasions
Volume 7 - 2024 | doi: 10.3389/ffgc.2024.1518972

On the look-out for a potential antagonist against Cryptostroma corticale -An insight through in vitro dual-culture studies

Provisionally accepted
Rebekka Schlößer Rebekka Schlößer 1,2,3*Steffen Bien Steffen Bien 1Johanna Bußkamp Johanna Bußkamp 1Gitta Jutta Langer Gitta Jutta Langer 1Ewald Johannes Langer Ewald Johannes Langer 3
  • 1 Northwest German Forest Research Institute, Göttingen, Germany
  • 2 Institute for the Sustainable Protection of Plants, Secondary Office Florence, Institute for Sustainable Plant Protection, National Research Council (CNR), Sesto Fiorentino (FI), Italy
  • 3 Department of Ecology, University Kassel, Kassel, Germany

The final, formatted version of the article will be published soon.

    In an effort to find a potential antagonist for Cryptostroma corticale, the causal agent of the sooty bark disease, different fungi previously isolated from Acer pseudoplatanus were tested in dual-culture antagonism assays with C. corticale. In total 102 fungal strains, mainly Ascomycota, were tested. Each potentially antagonistic strain was paired with three different strains of C. corticale. Four different tests were conducted to get a better understanding of the interactions between C. corticale and the potential antagonists. Test 1 was a dual-culture set up at room temperature with all 102 strains to get an overview of the interactions with C. corticale, as well as placing the potential antagonist on a Petri dish one week prior to C. corticale for a selection of fungi. For Test 2, only fungi which showed inhibition at distance in Test 1 were chosen and tested on different media at 25 °C, the optimal growing temperature of the pathogen. In Test 3, fungi showing signs of antagonism in Test 1 were tested against C. corticale in a 6:1 ratio of potential antagonist plugs to the C. corticale plug. For Test 4, the viability of C. corticale

    Keywords: fungal endophytes, Sooty bark disease, Sycamore maple, Antagonism, biocontrol

    Received: 29 Oct 2024; Accepted: 11 Dec 2024.

    Copyright: © 2024 Schlößer, Bien, Bußkamp, Langer and Langer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Rebekka Schlößer, Northwest German Forest Research Institute, Göttingen, Germany

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.