AUTHOR=Bérubé Jean A. , Allison Jeremy D. , Van Rooyen Kate , Hughes Cory , Gagné Patrick N. , Ochoa Isabelle , Sweeney Jon TITLE=Comparison of intercept trap fluids and aerial spore collectors to survey fungal spores JOURNAL=Frontiers in Forests and Global Change VOLUME=5 YEAR=2022 URL=https://www.frontiersin.org/journals/forests-and-global-change/articles/10.3389/ffgc.2022.953130 DOI=10.3389/ffgc.2022.953130 ISSN=2624-893X ABSTRACT=

Surveillance for early detection of non-native, invasive pathogens requires simple, sturdy, and easy-to-use collecting devices. In this study, we compared the fungal species detected in wet collection cups of Lindgren traps vs. those detected on slides with oiled cheesecloth as aerial spore collectors. DNA was extracted and amplified from both using the primers ITS1F and gITS7, and Illumina sequencing was used for the metabarcoding of fungi present in samples. In 90 samples, there were 1,277 fungal operational taxonomic units (OTUs). For fungal OTUs only detected by one collection method, insect traps had three times the number of fungal OTUs compared to slides, and this pattern persisted when analyses were restricted to pathogens and forest pathogens. Annually, thousands of insect traps are deployed in North America and the associated trap fluids have added value to forest disease research and monitoring.