AUTHOR=Ward Eric J. , Warren Jeffrey M. , McLennan David A. , Dusenge Mirindi E. , Way Danielle A. , Wullschleger Stan D. , Hanson Paul J. 

TITLE=Photosynthetic and Respiratory Responses of Two Bog Shrub Species to Whole Ecosystem Warming and Elevated CO2 at the Boreal-Temperate Ecotone

JOURNAL=Frontiers in Forests and Global Change

VOLUME=2

YEAR=2019

URL=https://www.frontiersin.org/journals/forests-and-global-change/articles/10.3389/ffgc.2019.00054

DOI=10.3389/ffgc.2019.00054

ISSN=2624-893X

ABSTRACT=<p>Peatlands within the boreal-temperate ecotone contain the majority of terrestrial carbon in this region, and there is concern over the fate of such carbon stores in the face of global environmental changes. The Spruce and Peatland Response Under Changing Environments (SPRUCE) facility aims to advance the understanding of how such peatlands may respond to such changes, using a combination of whole ecosystem warming (WEW; +0, 2.25, 4.5, 6.75, and 9°C) and elevated CO<sub>2</sub> (eCO<sub>2</sub>; +500 ppm) treatments in an intact bog ecosystem. We examined photosynthetic and respiration responses in leaves of two ericaceous shrub species–leatherleaf [<italic>Chamaedaphne calyculata</italic> (L.) Moench] and bog Labrador tea [<italic>Rhododendron groenlandicum</italic> (Oeder) Kron &amp; Judd]–to the first year of combined eCO<sub>2</sub> and WEW treatments at SPRUCE. We surveyed the leaf N content per area (<italic>N</italic><sub><italic>area</italic></sub>), net photosynthesis (<italic>A</italic><sub><italic>ST</italic></sub>) and respiration (<italic>R</italic><sub><italic>D</italic>25</sub>) at 25°C and 400 ppm CO<sub>2</sub> and net photosynthesis at mean growing conditions (<italic>A</italic><sub><italic>GR</italic></sub>) of newly emerged, mature and overwintered leaves. We also measured leaf non-structural carbohydrate content (<italic>NSC</italic>) in mature leaves. The effects of WEW and eCO<sub>2</sub> varied by season and species, highlighting the need to accommodate such variability in modeling this system. In mature leaves, we did not observe a response to either treatment of <italic>A</italic><sub><italic>ST</italic></sub> or <italic>R</italic><sub><italic>D</italic>25</sub> in <italic>R. groenlandicum</italic>, but we did observe a 50% decrease in <italic>A</italic><sub><italic>ST</italic></sub> of <italic>C. calyculata</italic> with eCO<sub>2</sub>. In mature leaves under eCO<sub>2</sub>, neither species had increased <italic>A</italic><sub><italic>GR</italic></sub> and both had increases in <italic>NSC</italic>, indicating acclimation of photosynthesis to eCO<sub>2</sub> may be related to source-sink imbalances of carbohydrates. Thus, productivity gains of shrubs under eCO<sub>2</sub> may be lower than previously predicted for this site by models not accounting for such acclimation. In newly emerged leaves, <italic>A</italic><sub><italic>ST</italic></sub> increased with WEW in <italic>R. groenlandicum</italic>, but not <italic>C. calyculata</italic>. Overwintered leaves exhibited a decrease in <italic>R</italic><sub><italic>D</italic>25</sub> for <italic>R. groenlandicum</italic> and in <italic>A</italic><sub><italic>ST</italic></sub> for <italic>C. calyculata</italic> with increasing WEW, as well as an increase of <italic>A</italic><sub><italic>GR</italic></sub> with eCO<sub>2</sub> in both species. Responses in newly emerged and overwintered leaves may reflect physiological acclimation or phenological changes in response to treatments.</p>