AUTHOR=Ferri Gianluigi , Buonavoglia Alessio , Farooq Muhammad , Festino Anna Rita , Ruffini Fausto , Paludi Domenico , Di Francesco Cristina E. , Vergara Alberto , Smoglica Camilla 

TITLE=Antibiotic resistance in Italian poultry meat production chain: a one-health perspective comparing antibiotic free and conventional systems from the farming to the slaughterhouse

JOURNAL=Frontiers in Food Science and Technology

VOLUME=3

YEAR=2023

URL=https://www.frontiersin.org/journals/food-science-and-technology/articles/10.3389/frfst.2023.1168896

DOI=10.3389/frfst.2023.1168896

ISSN=2674-1121

ABSTRACT=<p>In this study a multidisciplinary approach was applied in order to determine the diffusion of resistant bacteria and selected antibiotic resistance genes in antibiotic-free and conventional broiler farms. Litter samples coming from the two farming types and surface sponges obtained from carcasses at slaughterhouse level were screened by end-point PCR targeting specific resistance for tetracycline, ampicillin, sulfonamide, aminoglycoside, carbapenem, nitrofurantoin, vancomycin, quinupristin-dalfopristin, lincomycin, linezolid, chloramphenicol molecules. Microbiological investigations were conducted from the carcasses to determine phenotypical and genetic resistance patterns from pathogenic and commensal Gram-negative and Gram-positive strains. At farm level, <italic>cat</italic>A1, <italic>sul</italic>2, <italic>bla</italic><sub>TEM</sub> and <italic>aad</italic>A2 genes were amplified in all samples, while from carcasses the most representative genes were <italic>sul</italic>2, <italic>bla</italic><sub>TEM</sub>, along with the <italic>vat</italic>D, relative to quinupristin-dalfopristin resistance. Gram-negative isolates included <italic>Aeromonas</italic>, <italic>Salmonella</italic>, <italic>Proteus</italic> spp. And <italic>Escherichia coli</italic>, while the Gram-positive were represented by <italic>Enterococcus</italic> strains. Phenotypical and genetic analysis revealed multidrug resistance patterns in <italic>Salmonella</italic>, <italic>E. coli</italic> and <italic>Serratia</italic> isolates, followed by the <italic>Enterococcus</italic> species. The comparison between antibiotic-free and conventional farming systems showed some difference regarding the distribution of resistance genes at farm level but no significance was obtained comparing the phenotypical resistance profiles of bacterial strains from both groups of samples, suggesting a poor influence of farming model on the diffusion of antibiotic resistance in poultry meat production chain.</p>