AUTHOR=Trapp Judith , Gouveia Duarte , Almunia Christine , Pible Olivier , Degli Esposti Davide , Gaillard Jean-Charles , Chaumot Arnaud , Geffard Olivier , Armengaud Jean TITLE=Digging Deeper Into the Pyriproxyfen-Response of the Amphipod Gammarus fossarum With a Next-Generation Ultra-High-Field Orbitrap Analyser: New Perspectives for Environmental Toxicoproteomics JOURNAL=Frontiers in Environmental Science VOLUME=6 YEAR=2018 URL=https://www.frontiersin.org/journals/environmental-science/articles/10.3389/fenvs.2018.00054 DOI=10.3389/fenvs.2018.00054 ISSN=2296-665X ABSTRACT=
Taxon-specific proteins from sentinel species have been shown to be among the most relevant proteins to monitor the biological effects of anthropogenic contaminations. Shotgun proteomics allows high-throughput analysis of animal proteomes, but comprehensive coverage remains difficult to achieve due to the wide dynamic range. Here, the advantages of a new generation of mass analyser for environmental toxicoproteomics are documented through the identification of candidate biomarkers in a sentinel animal species for which the genome is as yet unsequenced. A label-free comparative proteomic study was performed on testis from the freshwater amphipod