AUTHOR=Shen Yuli , Kang Bohua , Lu Yi , Du Xinyuan , Qin Chuan , Li Jing , Zhao Zhaoer , Yu Ran , Shi Suan , Han Lujia 

TITLE=Production of optical pure L-lactic acid from Cabernet Sauvignon grape pomace by engineered Lactiplantibacillus plantarum

JOURNAL=Frontiers in Energy Research

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/energy-research/articles/10.3389/fenrg.2023.1228827

DOI=10.3389/fenrg.2023.1228827

ISSN=2296-598X

ABSTRACT=<p><italic>Cabernet Sauvignon</italic> grape pomace contains carbohydrates and various amino acids that could be used as substrates for lactic acid (LA) production. In this study, a mutant strain of <italic>L. plantarum</italic> with deletion of the D-lactate synthesis gene was developed and used to produce optical pure L-LA from grape pomace. The highest optical purity of the L-LA produced by this mutant strain was 99.61%. The direct bioconversion of the raw substrate showed a low LA yield. Several pretreatment methods were applied to improve the LA yield, including ball milling, hydrothermal, dilute acid pretreatment, alkaline pretreatment, and combined wet alkaline mechanical pretreatment. Due to the efficient delignification, alkaline pretreatment achieved the highest lactic acid yield of 96.26% at 15% solid loading, with corresponding LA concentration and volumetric productivity of 18.45 g/L and 2.30 g/L·h, respectively.</p>