AUTHOR=Yang Jing , Zhao Jian , Jiang Jianchun , Xu Hao , Zhang Ning , Xie Jingcong , Wei Min 

TITLE=Isolation and Characterization of Bacillus Sp. Capable of Degradating Alkali Lignin

JOURNAL=Frontiers in Energy Research

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/energy-research/articles/10.3389/fenrg.2021.807286

DOI=10.3389/fenrg.2021.807286

ISSN=2296-598X

ABSTRACT=<p>Alkali lignin-degrading <italic>Bacillus</italic> were isolated from forest soils in China and were identified as <italic>Bacillus subtilis</italic> TR-03 and <italic>Bacillus cereus</italic> TR-25 by 16S rDNA sequence analysis. Wherein TR-03 displayed optimal 26.72% alkali lignin (2 g/L) degradation at 7 days and 71.23% of Azure-B (0.01%) decolorization at 36 h of cultivation at 37°C. Ligninolytic enzyme analysis revealed that TR-03 was capable of depolymerizing alkali lignin effectively by the producing of lignin peroxidase and laccase, wherein higher laccase activity was cell-associated. At last, the physical and chemical changes of lignin <italic>via</italic> SEM and FTIR analysis was further observed to prove the lignin degradation by <italic>Bacillus subtilis</italic> TR-03.</p>