AUTHOR=Shi Kaiyi , Wang Jun 

TITLE=Research on a Mixed Culture Technique of the White Rot Fungi Effect of Extracellular Lignin Peroxidase on Lignite Liquefaction

JOURNAL=Frontiers in Energy Research

VOLUME=7

YEAR=2019

URL=https://www.frontiersin.org/journals/energy-research/articles/10.3389/fenrg.2019.00133

DOI=10.3389/fenrg.2019.00133

ISSN=2296-598X

ABSTRACT=<p>White rot fungi mixed culture technology was applied in order to improve the efficiency of lignite liquefaction. In this paper, 15 fungi were used to liquefy Zhaotong lignite. The results showed that there was a good linear relationship between the liquefaction effect and lignin peroxidase (LiP). Since the low activity of LiP is the barrier in real practice, five strains with the highest LiP activity for a combinatorial culture suggested that a mixed strains culture is an effective way to improve LiP activity and the liquefaction effect, and the average enzyme activity was 76.28 U/L. The single strain, however, was only 19.24 U/L. Among 10 mixed culture strains, four of them showed obvious synergy, and the one with maximum enzyme activity had increased by 6.7 times its original size. The analysis of lignite and liquefaction products using <sup>13</sup>C NMR showed that the aromatic carbon (<italic>f</italic><sub>a</sub>) decreased while the phenol or aromatic ether carbon (<inline-formula><mml:math id="M1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msubsup><mml:mrow><mml:mi>f</mml:mi></mml:mrow><mml:mrow><mml:mtext>a</mml:mtext></mml:mrow><mml:mrow><mml:mtext>P</mml:mtext></mml:mrow></mml:msubsup></mml:math></inline-formula>) and oxygen-linked fatty carbon (<inline-formula><mml:math id="M2" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msubsup><mml:mrow><mml:mi>f</mml:mi></mml:mrow><mml:mrow><mml:mtext>al</mml:mtext></mml:mrow><mml:mrow><mml:mtext>O</mml:mtext></mml:mrow></mml:msubsup></mml:math></inline-formula>) increased, which proved that LiP had undergone ring-opening, oxidation, and other reactions during lignite liquefaction.</p>