Editorial: Steroids and the Brain -Volume II

Takayoshi Ubuka ^1* Vance L. Trudeau ²

• ¹ Medicine, International Cancer Laboratory Co., Ltd., Tokyo, Selangor, Japan
• ² University of Ottawa, Ottawa, Ontario, Canada

elevated levels of urinary total testosterone, urinary dehydroepiandrosterone, and free testosterone in ASD individuals. In particular, dehydroepiandrosterone levels were significantly elevated in ASD males.Depression is more common in people with epilepsy. In a study that used the Zung Self-Reported Depression Scale, depression occurred in 26.9% of patients with epilepsy compared with 9.7% in the control cohort (9). Women with epilepsy face particular challenges with seizures and anti-seizure medications (ASMs) (10). Ogunjimi et al. investigated the association of hormones related to reproduction, including sex steroids, ASM, and depression among epileptic women. Blood samples were collected during the luteal phase (LP) and follicular phase (FP). There were statistical differences between cases and controls in testosterone and prolactin. Testosterone, FP folliclestimulating hormone (FSH), FP estradiol, LP FSH, LP progesterone, and LP prolactin were associated with depression. Differences in various hormonal levels in epileptic women were also found by using different ASMs, such as carbamazepine and levetiracetam.Why are there sex differences in the responses to stressors? Previous studies characterized the roles of androgens in mediating the sex differences in neuroendocrine and behavioral stress responses (11). Amaya et al. investigated whether glucocorticoid signaling in the brain may be modulated by androgens. They compared the expression of glucocorticoid receptor (GR) target genes in the brain regions where GR and androgen receptor (AR) are co-expressed after chronic treatment with corticosterone, dihydrotestosterone, combination of both, or corticosterone in combination with the AR antagonist enzalutamide. Their results showed that androgens affected glucocorticoid signaling only in the prefrontal cortex and the substantia nigra, and not in the hypothalamus, hippocampus, and ventral tegmental area. This study highlights the role of the prefrontal cortex and the substantia nigra in mediating the sex differences in the responses to stressors.Inflammation in the brain and periphery have recently been recognized as playing critical roles in the development and progression of neurological and psychiatric disorders (12). Neurosteroids such as pregnenolone and allopregnanolone emerged as regulators of inflammatory and neuroinflammatory responses (13). Previous studies demonstrated the inhibitory effect of allopregnanolone on the activation of inflammatory toll-like receptor 4 (TLR4) signaling in RAW264.7 macrophages and the brain of alcohol-preferring rats. Balan et al. investigated the impact of allopregnanolone on the levels of interleukin-10, an anti-inflammatory mediator cytokine, and activation of the TRIF-dependent endosomal TLR4 pathway. Their results demonstrate allopregnanolone enhancement of the endosomal TRL4-TRIF anti-inflammatory signals and elevations of interleukin-10 in the male alcohol-preferring rat brain but not in females.The rate limiting step for the synthesis of the estrogens involves aromatase (Cyp19a1) that respectively converts the androgens androstenedione and testosterone into the bioactive estrogens, estrone and estradiol. Teleosts, the bony fishes, have two paralogous aromatase genes, cyp19a1a and cyp19a1b, that are highly expressed in the ovary and the brain, respectively. It was recently shown that cyp19a1b mutant female zebrafish that have significantly lower estradiol levels in the brain also have altered female sexual behavior (14). Although brain aromatase is constitutively expressed in neurons in mice, cyp19a1b is exclusively expressed in radial glial cells in teleosts. Shaw et al. investigated the mechanistic pathways of cyp19a1b mutant female zebrafish in the disruption of female reproductive behavior. They found that delayed oviposition in female mutant cyp19a1b -/- zebrafish is linked to impaired arginine vasopressin (also known and vasotocin in teleosts) signaling in the brain. This study also suggests that female behavioral phenotype of cyp19a1b -/-zebrafish is a