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ORIGINAL RESEARCH article
Front. Endocrinol.
Sec. Diabetes: Molecular Mechanisms
Volume 16 - 2025 | doi: 10.3389/fendo.2025.1536639
Identification of Novel Target genes in Exaggerated Cardiac Remodeling Following Myocardial Infarction in Diabetes
Provisionally accepted- 1 Beijing Anzhen Hospital, Capital Medical University, Chaoyang District, Beijing, China
- 2 Beijing Children’s Hospital, Capital Medical University, Beijing, Beijing Municipality, China
- 3 China-Japan Friendship Hospital, Beijing, Beijing Municipality, China
- 4 Peking Union Medical College Hospital (CAMS), Beijing, Beijing Municipality, China
- 5 Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China
Diabetes mellitus is a significant risk factor for myocardial infarction (MI). Our study investigates the exacerbating effect of diabetes mellitus on MI using high-throughput sequencing technology.Type 2 diabetes mellitus mouse model was developed through a high-sugar and high-fat diet (HFD), followed by MI surgery. Four weeks post-surgery, cardiac function was evaluated via echocardiography, and cardiac pathology was examined using Masson's trichrome and wheat germ agglutinin staining. High-throughput sequencing identified differentially expressed mRNAs and long non-coding RNAs (LncRNAs) in diabetic mice with MI. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, along with LncRNA-target-gene analysis, were performed. Validation in human samples of diabetic patients with STEMI confirmed the influence of HFD on the expression of specific genes. The results demonstrate that diabetes significantly impairs cardiac function, exacerbates cardiac fibrosis and hypertrophy. In addition, our extensive examination of human samples has conclusively demonstrated that diabetes significantly modulates the expression of genes (Rapgef5 and Ing1) within the cardiac tissue of individuals afflicted with STEMI, underscoring the intricate interplay between these conditions. In addition, we have found that Rapgef5 and Ing1 are involved in diabetes-mediated cardiomyocyte apoptosis and proliferation following myocardial infarction. These findings elucidate the molecular mechanisms by which diabetes worsens post-MI cardiac remodeling.
Keywords: diabetes, Myocardial Infarction, lncRNA, mRNA, cardiomycytes
Received: 03 Dec 2024; Accepted: 25 Feb 2025.
Copyright: © 2025 Duan, Zhang, Xia, Li, Liu and Du. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Demin Liu, Second Hospital of Hebei Medical University, Shijiazhuang, 050000, Hebei Province, China
Yunhui Du, Beijing Anzhen Hospital, Capital Medical University, Chaoyang District, 100029, Beijing, China
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