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ORIGINAL RESEARCH article
Front. Endocrinol.
Sec. Reproduction
Volume 16 - 2025 |
doi: 10.3389/fendo.2025.1534648
Single-cell dual-omics reveals translational and transcriptional landscapes and regulations in oocytes from ovarian endometriosis patients
Provisionally accepted- The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
A significant proportion of women in their reproductive years are afflicted by endometriosis. And one of the major contributing factors to infertility linked to ovarian endometriosis is thought to be oocyte quality. The precise molecular mechanisms are still unknown. Furthermore, translatome may describe more clearly of the molecular behaviors in oocytes because of transcriptional silence. Therefore, we first conducted single-cell transcriptome and translatome sequencing to characterize the underlying processes of reduced oocyte quality in women with ovarian endometriosis. Translational analysis revealed 2480 differentially expressed genes in oocytes from ovarian endometriosis patients. CDK1, CCNB1, and CHEK1, all linked to meiosis, were among the downregulated genes. CYC1, COX5B, NDUFB8, and UQCRQ were among the up-regulated genes linked to oxidative phosphorylation. Additionally, we first reported that oocytes from patients with ovarian endometriosis exhibit abnormal control of RNA splicing. Furthermore, we revealed a distinct translation pattern in patients with ovarian endometriosis. In conclusions, the molecular characteristics and possible processes in oocytes linked to poor quality in patients with ovarian endometriosis are shown by this study. It provides insight into the infertility linked to ovarian endometriosis and identifies possible targets for treatment.
Keywords: Oocyte quality, ovarian endometriosis, Oocyte meiosis, Oxidative Stress, RNA Splicing
Received: 26 Nov 2024; Accepted: 23 Jan 2025.
Copyright: © 2025 Diao, Huang, Xiang, Zhuang, Liang, Liang and Zeng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Haitao Zeng, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
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