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ORIGINAL RESEARCH article
Front. Endocrinol.
Sec. Reproduction
Volume 16 - 2025 | doi: 10.3389/fendo.2025.1510114
High sperm DNA Stainability (HDS) might not be an accurate predictive indicator of male fertility and assisted reproductive technology outcomes
Provisionally accepted- Reproductive and Genetic Center, Suzhou Municipal Hospital, Suzhou, China
The clinical need for assisted reproduction continued to increase, so did the need for predictive markers of assisted reproductive technology (ART) outcomes. Among all the markers, sperm DNA integrity was paid more and more attention in the assessment of male fertility in recent years, but its clinical value remains still in doubt. Methods: We conducted a retrospective cohort study. Couples coming to our reproductive center were retrospectively enrolled, and semen and assisted reproductive technology parameters were assessed. Sperm DNA integrity was analyzed using a flow cytometric method. Statistics were analyzed to investigate the relationship of this on semen quality and ART outcomes. Results: DNA fragmentation index (DFI) DFI was affected by abstinence days and age, and was a directly correlated with sperm quality parameters (p<0.001). Meanwhile high sperm DNA stainability (HDS) HDS showed an unexplainable negative correlation with abstinence days (p<0.05), age (p<0.001) and body mass index (p<0.01).For sperm quality parameters, HDS showed a similar relevance besides abnormal sperm head morphology (p<0.001). Embryo cleavage and implant implantation rates were significantly negatively related to DFI in fresh intracytoplasmic sperm injection cycles (p<0.01) while HDS showed a positive relationship with high quality embryo rate (p<0.05). For final outcomes, only the live birth rate from fresh intracytoplasmic sperm injection cycles was positively correlated with DFI which is meaningless (p<0.05). Conclusions: HDS might not be an appropriate marker for male fertility and further studies are needed to identify the efficiency of SDF in clinical practice.
Keywords: Infertility, SCSA, DFI, HDS, ART outcomes
Received: 17 Oct 2024; Accepted: 11 Feb 2025.
Copyright: © 2025 Shen, Zhang, Wang, Fu, Yang and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jiaxiong Wang, Reproductive and Genetic Center, Suzhou Municipal Hospital, Suzhou, China
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