Differential lipids in euthyroid pregnant women with positive TPOAb and its correlation with clinical parameters

Xinxin Chen Yuxin Qin Wang Qingyao Ying Wu Huanhuan Zang Xiangguo Cong Qiong Shen Lei Chen ^*

• Suzhou Municipal Hospital, Suzhou, China

Pregnant women with subclinical hypothyroidism or clinical hypothyroidism often exhibit lipid metabolism disorders and are correlated with adverse pregnant outcomes. It was suggested that isolated positive thyroid peroxidase antibody (TPOAb) served as a risk factor for adverse outcomes. However, little was known about the lipid metabolism profile in pregnant women with isolated positive TPOAb.The purpose of this prospective observational study was to investigate lipid profile changes by using untargeted liquid chromatography-mass spectrometry (LC-MS) and to explore their associations with clinical parameters. A total of 90 pregnant women in the first trimester were enrolled in the analysis: 46 were TPOAb-positive euthyroid women, and 44 were healthy controls. A total of 1238 lipid molecules were identified. These molecules belonging to 46 lipid classes and 5 lipid categories (fatty acyl (FA), glycerophospholipid (GP), glycerolipid (GL), sphingolipid (SP), and sterol lipid (ST)). A total of 202 differential lipid molecules were screened between the two groups. KEGG pathway enrichment analysis revealed that the differentially expressed lipids participate in multiple pathways, such as Thermogenesis, Regulation of lipolysis, PD-L1 expression and PD-1 checkpoint pathway in cancer, Neurotrophin signaling pathway, Natural killer cell mediated cytotoxicity, NF-kappa B signaling pathway, Insulin resistance, Glycerophospholipid metabolism, Choline metabolism in cancer, Adipocytokine signaling pathway, Fat digestion and absorption, AGE-RAGE signaling pathway in diabetic complications, Diabetic cardiomyopathy, the Th1/Th2/Th17 cell differential pathway.