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ORIGINAL RESEARCH article

Front. Endocrinol.
Sec. Renal Endocrinology
Volume 15 - 2024 | doi: 10.3389/fendo.2024.1404075
This article is part of the Research Topic Unraveling Ischemia-Reperfusion Injury: Advances in Injury Mechanisms and Treatment Targets View all articles

Metabolomic and Transcriptomic Insights into the Mechanisms of Renal Ischemia-Reperfusion Injury Progression

Provisionally accepted
Wanyi Li Wanyi Li 1Xiaoqin Liu Xiaoqin Liu 1*Lingling Gan Lingling Gan 1Honglin Li Honglin Li 1*Jiawei Zeng Jiawei Zeng 1*Bei Xu Bei Xu 1,2*
  • 1 Mianyang Central Hospital, Mianyang, China
  • 2 NHC Key Laboratory of Nuclear Technology Medical Transformation, Mianyang Central Hospital, Mianyang, China

The final, formatted version of the article will be published soon.

    Renal ischemia-reperfusion injury (IRI) is an important cause of acute kidney injury (AKI). However, the pathophysiological changes and mechanisms during IRI-AKI progression remain unclear. This study aims toinvestigate the potential mechanisms in the progression of IRI-AKI by integrating 2 metabolomics and transcriptomics data, providing a reference for the subsequent identification of biomarkers and therapeutic targets. IRI-AKI rat models with 30 min of ischemia and 24 h or 72 h of reperfusion surgery simulating the progression of AKI were established. Compared to the control group underwent sham surgery (NC group), most of the differentially expressed metabolites (DEMs) in IRI-AKI 24 h and IRI-AKI 72 h decreased, mainly including amino acids, organic acids, and carnitines. Additionally, we found that DEMs were mainly enriched in amino acid-related pathways, among which valine, leucine, and isoleucine biosynthesis were dramatically altered in all comparisons. Transcriptomics revealed that differentially expressed genes (DEGs) were primarily involved in amino acid, lipid, and fatty acid metabolism. By integrating metabolomics and transcriptomics, we found valine, leucine, and isoleucine biosynthesis play key roles in IRI-AKI development. Our findings concluded that valine, leucine, and isoleucine pathways are hubs that potentially connect transcriptomes to metabolomes, providing new insights regarding the pathogenesis of IRI-AKI and its potential biomarkers and therapeutic strategies.

    Keywords: Acute Kidney Injury, Metabolomics, Transcriptomics, ischemia-reperfusion injury, biomarkers

    Received: 20 Mar 2024; Accepted: 02 Dec 2024.

    Copyright: © 2024 Li, Liu, Gan, Li, Zeng and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xiaoqin Liu, Mianyang Central Hospital, Mianyang, China
    Honglin Li, Mianyang Central Hospital, Mianyang, China
    Jiawei Zeng, Mianyang Central Hospital, Mianyang, China
    Bei Xu, Mianyang Central Hospital, Mianyang, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.