AUTHOR=Lopez-Tello Jorge , Sferruzzi-Perri Amanda N. 

TITLE=Characterization of placental endocrine function and fetal brain development in a mouse model of small for gestational age

JOURNAL=Frontiers in Endocrinology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2023.1116770

DOI=10.3389/fendo.2023.1116770

ISSN=1664-2392

ABSTRACT=<p>Conditions such as small for gestational age (SGA), which is defined as birthweight less than 10<sup>th</sup> percentile for gestational age can predispose to neurodevelopmental abnormalities compared to babies with normal birthweight. Fetal growth and birthweight depend on placental function, as this organ transports substrates to the developing fetus and it acts as a source of endocrine factors, including steroids and prolactins that are required for fetal development and pregnancy maintenance. To advance our knowledge on the aetiology of fetal growth disorders, the vast majority of the research has been focused on studying the transport function of the placenta, leaving practically unexplored the contribution of placental hormones in the regulation of fetal growth. Here, using mice and natural variability in fetal growth within the litter, we compared fetuses that fell on or below the 10<sup>th</sup> percentile (classified as SGA) with those that had adequate weight for their gestational age (AGA). In particular, we compared placental endocrine metabolism and hormone production, as well as fetal brain weight and expression of developmental, growth and metabolic genes between SGA and AGA fetuses. We found that compared to AGA fetuses, SGA fetuses had lower placental efficiency and reduced capacity for placental production of hormones (e.g. steroidogenic gene <italic>Cyp17a1</italic>, prolactin <italic>Prl3a1</italic>, and pregnancy-specific glycoproteins <italic>Psg21</italic>). Brain weight was reduced in SGA fetuses, although this was proportional to the reduction in overall fetal size. The expression of glucose transporter 3 (<italic>Slc2a3</italic>) was reduced despite the abundance of AKT, FOXO and ERK proteins were similar. Developmental (<italic>Sv2b</italic> and <italic>Gabrg1</italic>) and microglia genes (<italic>Ier3</italic>), as well as the pregnancy-specific glycoprotein receptor (<italic>Cd9)</italic> were lower in the brain of SGA versus AGA fetuses. In this mouse model of SGA, our results therefore demonstrate that placental endocrine dysfunction is associated with changes in fetal growth and fetal brain development.</p>