AUTHOR=Wang Bin , Cui Aijun , Xu Yongjiang , Zhang Yaxing , Jiang Yan , Liu Xuezhou 

TITLE=Food deprivation differentially modulates gene expression of LPXRFa and kisspeptin systems in the brain-pituitary axis of half-smooth tongue sole (Cynoglossus semilaevis)

JOURNAL=Frontiers in Endocrinology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2023.1099832

DOI=10.3389/fendo.2023.1099832

ISSN=1664-2392

ABSTRACT=<p>LPXRFa, also known as gonadotropin-inhibitory hormone (GnIH), and kisspeptin (Kiss) are two major hypothalamic peptides that modulate the reproductive axis of vertebrates, including teleosts. However, little information is available regarding the actions of nutritional status on the regulation of these two neuroendocrine systems in fish. Herein, we assessed the effects of starvation and refeeding on the expression of <italic>lpxrfa</italic>, <italic>kiss2</italic> and their receptors (<italic>lpxrfa-r</italic> and <italic>kiss2r</italic> respectively) at the brain-pituitary level of half-smooth tongue sole (<italic>Cynoglossus semilaevis</italic>). Food deprivation for 4 weeks induced a rise in brain <italic>lpxrfa</italic> as well as brain and pituitary <italic>lpxrfa-r</italic> mRNA levels, and refeeding restored brain <italic>lpxrfa</italic> and <italic>lpxrfa-r</italic> expression back to normal. However, pituitary <italic>lpxrfa-r</italic> mRNA levels still remained high after 1 week of refeeding. Neither <italic>lpxrfa</italic> nor <italic>kiss2</italic> transcripts in the pituitary were altered by fasting, but their mRNA levels increased significantly after 1 week of refeeding, and declined back to the control levels after 2 weeks of refeeding. None of brain <italic>kiss2</italic> and <italic>kiss2r</italic> along with pituitary <italic>kiss2r</italic> transcripts were modified by the nutritional status. In summary, our results revealed an interaction between energy status and the elements of LPXRFa and Kiss systems in the brain-pituitary axis of half-smooth tongue sole. Food deprivation and refeeding differentially regulated the two systems, which provided additional evidence for the involvement of the LPXRFa and Kiss systems in the regulation of reproduction by energy balance in non-mammalian species.</p>