AUTHOR=Chang Wei-Wei , Wen Li-Ying , Zhang Liu , Tong Xin , Jin Yue-Long , Chen Gui-Mei 

TITLE=Association of rs2910164 in miR-146a with type 2 diabetes mellitus: A case–control and meta-analysis study

JOURNAL=Frontiers in Endocrinology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2022.961635

DOI=10.3389/fendo.2022.961635

ISSN=1664-2392

ABSTRACT=<sec><title>Objective</title><p>Several studies have shown that miR-146a rs2910164 (C &gt; G) is associated with type 2 diabetes mellitus (T2DM) susceptibility, but the results are still controversial. This study is divided into two parts, and one is to explore the relationship between miR-146a rs2910164 polymorphism and the genetic susceptibility of T2DM in Chinese Han population. Second, a meta-analysis on the basis of a larger sample size was used to determine whether this is a susceptibility gene for T2DM.</p></sec><sec><title>Methods</title><p>A case–control study including 574 T2DM patients and 596 controls was used to evaluate the association of miR-146a rs2910164 polymorphism with the risk of T2DM in Chinese Han People. Then, we systematically searched studies investigating the correlation between miR-146a rs2910164 polymorphism and T2DM susceptibility published before April 2022 from PubMed, Web of Science, Wanfang, and China National Knowledge Infrastructure database, and a meta-analysis including six studies was carried out. The results were expressed by odds ratio (<italic>OR</italic>) and its 95% confidence interval (95% CI).</p></sec><sec><title>Results</title><p>In a case–control study, we found that there were no statistical differences in genotype frequencies between T2DM and control group. Subgroup analysis showed that, compared with the CC genotype, CG + GG genotype was associated with a decreased risk of T2DM in the subgroup of individuals ≥ 65 years old (<italic>OR</italic> = 0.75; 95% CI: 0.58–0.98; <italic>P</italic><sub>adjusted</sub> = 0.032) and BMI &lt; 18.5 (<italic>OR</italic> = 0.16; 95% CI: 0.03–0.89; <italic>P</italic><sub>adjusted</sub> = 0.037). In overall meta-analysis, significant heterogeneity was detected. No significant association between miR-146a rs2910164 polymorphism and T2DM was observed in all genetic models under random effects models. Subgroup analysis revealed that there was a significant difference in genotype frequencies between the T2DM and control group in recessive model (CC vs. CG + GG: <italic>OR</italic> = 1.79; 95% CI: 1.08–2.96; <italic>P<sub>Q</sub></italic> = 0.307, <italic>I</italic><sup>2</sup> = 4.0%) and homozygote model (CC vs. GG: <italic>OR</italic> = 1.79; 95% CI: 1.07–3.00; <italic>P<sub>Q</sub></italic> = 0.216, <italic>I</italic><sup>2</sup> = 34.7%) in Caucasians.</p></sec><sec><title>Conclusion</title><p>The results of our study demonstrate that the miR-146a rs2910164 polymorphism might have ethnicity-dependent effects in T2DM and may be related to T2DM susceptibility in Caucasians.</p></sec>