AUTHOR=Ye Haowen , Wang Ruxin , Wei Jinjing , Wang Ying , Zhang Xiaofang , Wang Lihong 

TITLE=Bioinformatics Analysis Identifies Potential Ferroptosis Key Gene in Type 2 Diabetic Islet Dysfunction

JOURNAL=Frontiers in Endocrinology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2022.904312

DOI=10.3389/fendo.2022.904312

ISSN=1664-2392

ABSTRACT=<sec><title>Background</title><p>Islet β cells dysfunction (IBCD) is a cortical component in pathogenesis of type 2 diabetic mellitus (T2DM). However, the relationship of ferroptosis and IBCD remains unknown. This study was aimed to screen potential ferroptosis key genes to reveal latent physiological and pathological process of IBCD in T2DM.</p></sec><sec><title>Methods</title><p>Firstly, T2DM key genes were screened by combining with differentially expressed genes (DEGs) analysis and WGCNA. Then, ferroptosis-related genes (FRGs) in IBCD of T2DM were identified by taking the intersection between T2DM key genes and FRGs. Finally, T2DM-FRGs were validated in another T2DM dataset as well as islet single-cell RNA sequencing dataset and the miRNA regulated T2DM-FRG was predicted by using four miRNA databases.</p></sec><sec><title>Results</title><p>89 T2DM key genes were identified between DEGs and WGCNA. Then, 3 T2DM-FRGs were screened by taking the intersection of T2DM key genes and FRGs, namely <italic>ITGA6</italic>, <italic>MGST1</italic> and <italic>ENO2</italic>. At last, <italic>MGST1</italic> were validated as the T2DM-FRG in another T2DM islet issues dataset and islet single-cell RNA sequencing dataset.</p></sec><sec><title>Conclusion</title><p><italic>MGST1</italic> may be the potential ferroptosis key gene of IBCD in T2DM.</p></sec>