AUTHOR=Ding Le-Xi , Zhang Jing , Yang Si-Si , Wu Jin , Su Tong , Wang Wei-Ming 

TITLE=Heat Shock Proteins 70 Regulate Cell Motility and Invadopodia-Associated Proteins Expression in Oral Squamous Cell Carcinoma

JOURNAL=Frontiers in Endocrinology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2022.890218

DOI=10.3389/fendo.2022.890218

ISSN=1664-2392

ABSTRACT=<sec><title>Background</title><p>Many studies have shown that diabetes is often closely related to oral squamous cell carcinoma (OSCC) occurrence and metastasis. Heat shock protein 70 (Hsp70) is a molecular chaperone related to diabetes complications. This study aims to investigate the role of Hsp70 in OSCC in expression of invadopodia-associated proteins.</p></sec><sec><title>Methods</title><p>The expressions and correlation of HSP70, Hif1α, MMP2, MMP14, and cortactin were examined using bioinformatics analysis and verified by OSCC tissue microarrays. Assay <italic>in vitro</italic> was performed to analyze cell migration capacity after treatment with or without the HSP70 inhibitor.</p></sec><sec><title>Results</title><p>The expressions of invadopodia-associated proteins were enhanced in OSCC tissues compared with paracarcinoma tissues and partially correlated with HSP70. Inhibiting HSP70 significantly decreased the cell viability, proliferation, and migration of OSCC cells.</p></sec><sec><title>Conclusions</title><p>HSP70 may be involved in invadopodia-associated proteins in OSCC cells, which provides a promising method for treatment of OSCC metastasis.</p></sec>