AUTHOR=Du Runyu , Li Ling , Wang Yanjun 

TITLE=N6-Methyladenosine-Related Gene Signature Associated With Monocyte Infiltration Is Clinically Significant in Gestational Diabetes Mellitus

JOURNAL=Frontiers in Endocrinology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2022.853857

DOI=10.3389/fendo.2022.853857

ISSN=1664-2392

ABSTRACT=<sec><title>Objective</title><p>The objective of this study was to reveal the potential crosstalk between immune infiltration and N<sup>6</sup>- methyladenosine (m<sup>6</sup>A) modification in the placentas of patients with gestational diabetes mellitus (GDM), and to construct a model for the diagnosis of GDM.</p></sec><sec><title>Methods</title><p>We analyzed imbalanced immune infiltration and differentially expressed m<sup>6</sup>A-related genes (DMRGs) in the placentas of patients with GDM, based on the GSE70493 dataset. An immune-related DMRG signature, with significant classifying power and diagnostic value, was identified using a least absolute shrinkage and selection operator (LASSO) regression. Based on the selected DMRGs, we developed and validated a nomogram model using GSE70493 and GSE92772 as the training and validation sets, respectively.</p></sec><sec><title>Results</title><p>Infiltration of monocytes was higher in GDM placentas than in control samples, while the infiltration of macrophages (M1 and M2) in GDM placentas was lower than in controls. A total of 14 DMRGs were strongly associated with monocyte infiltration, seven of which were significant in distinguishing patients with GDM from normal controls. These genes were <italic>CD81</italic>, <italic>CFH</italic>, <italic>FABP5</italic>, <italic>GBP1</italic>, <italic>GNG11</italic>, <italic>IL1RL1</italic>, and <italic>SLAMF6</italic>. The calibration curve, decision curve, clinical impact curve, and receiver operating characteristic curve showed that the nomogram recognized GDM with high accuracy in both the training and validation sets.</p></sec><sec><title>Conclusions</title><p>Our results provide clues that crosstalk between m<sup>6</sup>A modification and immune infiltration may have implications in terms of novel biomarkers and therapeutic targets for GDM.</p></sec>