AUTHOR=Song Linyi , Yu Jin , Zhang Danying , Li Xi , Chen Lu , Cai Zailong , Yu Chaoqin 

TITLE=Androgen Excess Induced Mitochondrial Abnormality  in Ovarian Granulosa Cells in a Rat Model of Polycystic Ovary Syndrome

JOURNAL=Frontiers in Endocrinology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2022.789008

DOI=10.3389/fendo.2022.789008

ISSN=1664-2392

ABSTRACT=<sec><title>Background</title><p>Androgen excess could profoundly lead to follicular dysplasia or atresia, and finally result in polycystic ovary syndrome (PCOS); however, the exact mechanism remains to be fully elucidated.</p></sec><sec><title>Methods</title><p>PCOS model rats were induced by dehydroepiandrosterone, and their fertility was assessed. The ovarian granulosa cells (GCs) from matured follicles of PCOS model rats were collected and identified by immunofluorescence. The mitochondrial ultrastructure was observed by transmission electron microscope and the mitochondrial function was determined by detecting the adenosine triphosphate (ATP) content and <italic>mtDNA</italic> copy number. Besides, the expressions of respiratory chain complexes and ATP synthases in relation to mitochondrial function were analyzed.</p></sec><sec><title>Results</title><p>The PCOS model rats were successfully induced, and their reproductive outcomes were obviously adverse. The GCs layer of the ovarian was apparently cut down and the mitochondrial ultrastructure of ovarian GCs was distinctly destroyed. The ATP content and <italic>mtDNA</italic> copy number of ovarian GCs in PCOS model rats were greatly reduced, and the expressions of <italic>NDUFB8</italic> and <italic>ATP5j</italic> were significantly down-regulated without obvious deletion of <italic>mtDNA</italic> 4834-bp.</p></sec><sec><title>Conclusions</title><p>Androgen excess could damage mitochondrial ultrastructure and function of GCs in rat ovary by down-regulating expression of <italic>NDUFB8</italic> and <italic>ATP5j</italic> in PCOS.</p></sec>