AUTHOR=Liu Yanli , Shen Junhan , Zhang Yuchao , Peng Rui , Zhao Junliang , Zhou Pengfei , Yang Rujing , Guan Yichun 

TITLE=Controlled ovarian hyperstimulation parameters are not associated with de novo chromosomal abnormality rates and clinical pregnancy outcomes in preimplantation genetic testing

JOURNAL=Frontiers in Endocrinology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2022.1080843

DOI=10.3389/fendo.2022.1080843

ISSN=1664-2392

ABSTRACT=<sec><title>Objective</title><p>This study aimed to determine whether controlled ovarian hyperstimulation (COH) parameters influence the incidence of <italic>de novo</italic> chromosomal abnormalities (&gt; 4 Mb) in blastocysts and, thus, clinical pregnancy outcomes in preimplantation genetic testing (PGT).</p></sec><sec><title>Methods</title><p>Couples who underwent preimplantation genetic testing for structural chromosome rearrangements (PGT-SR) and monogenic disorders (PGT-M) were included in this study. The relationships of maternal age, paternal age, stimulation protocol, exogenous gonadotropin dosage, duration of stimulation, number of oocytes retrieved and estradiol (E<sub>2</sub>) levels on human chorionic gonadotropin (hCG) trigger day with the incidence of <italic>de novo</italic> chromosomal abnormalities were assessed. Blastocysts were biopsied, and nuclear DNA was sequenced using next-generation sequencing (NGS). Clinical pregnancy outcomes after single euploid blastocyst transfers under different COH parameters were assessed. </p></sec><sec><title>Results</title><p>A total of 1,710 and 190 blastocysts were biopsied for PGT-SR and PGT-M, respectively. The rate of <italic>de novo</italic> chromosomal abnormalities was found to increase with maternal age (<italic>p&lt;</italic> 0.001) and paternal age (<italic>p</italic> = 0.019) in the PGT-SR group. No significant differences in the incidence of <italic>de novo</italic> chromosomal abnormalities were seen for different maternal or paternal age groups between the PGT-SR and PGT-M groups (<italic>p</italic> &gt; 0.05). Stratification analysis by gonadotropin dosage, stimulation protocol, duration of stimulation, number of retrieved oocytes and E<sub>2</sub> levels on hCG trigger day revealed that <italic>de novo</italic> chromosomal abnormalities and clinical pregnancy outcomes were not correlated with COH parameters after adjusting for various confounding factors.</p></sec><sec><title>Conclusion</title><p>The rate of <italic>de novo</italic> chromosomal abnormalities was found to increase with maternal or paternal age. COH parameters were found to not influence the incidence of <italic>de novo</italic> chromosomal abnormalities or clinical pregnancy outcomes.</p></sec>